Sequences from confirmation amplicon


Confirmation sequences for line 958E02 and hit At4g31480 are shown.
Line ID 958E02
Locus At4g31480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15265246
Primers used for PCR 24sw - 8474, show primer details
   
Sequence Name 54-K116654-0022-958-E02C-24sw
GenBank Accession LN508601 [GenBank]
Sequencing primer gene specific primer, 24sw: 5'-GTGATAGGCAATACTCCCCAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K116654-0022-958-E02C-24sw
ATCTGATGAACGTGTCCATAACCTTGTGATGATTGAAACTCTTAACTTGGGATTTGTTTC
TATTATCTCTCTAACAAAAACAACCACGTCAAGAGCTGAAGCCACATTGCTATCTCCCAA
GAAGTCCATCAGAAGGTGGACCACTGTGCTTGCAACTTCGGGGAACTTCACTGCACAAGC
GTGGATGGCTTGAATAAGCATTTGTCTGTACTCCCCATTCTTCTCAAGTTCTCCACTCTG
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCANTTATTATAGT
   
Sequence Name 29-K116653-0022-958-E02C-8409
GenBank Accession LN499448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116653-0022-958-E02C-8409
CTTGAGAAGATGGGGAGTACAGACAAATGCTTATTCAAGCCACCTTTACTTGTGCAGTGA
AGTTCCCCGAAGTTGCAAGCACAGTGGTCCACCTTCTGATGGACTTCTTGGGAGATAGCA
ATGTGGCTTCAGCTCTTGACGTGGTTGTTTTTGTTAGAGAGATAATAGAAACAAATCCCA
AGTTAAGAGTTTCAATCATCACAAGGTTATTGGACACGTTCTATCAGATCCGAGCAGGAA
AGGTCTGCCCTTGTGCCCTTTGGATAATTGGGGAGTATGGCCTATCAC

Last Updated on 06.12.2018 16:11