Sequences from confirmation amplicon


Confirmation sequences for line 960C11 and hit At1g72540 are shown.
Line ID 960C11
Locus At1g72540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27316900
Primers used for PCR 61tv - 8474, show primer details
   
Sequence Name 18-K117142-0022-960-C11C-61tv
GenBank Accession LN494480 [GenBank]
Sequencing primer gene specific primer, 61tv: 5'-ATTTAGCAGAAGCAACCATGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117142-0022-960-C11C-61tv
AATTCATTACAACTTACAAGTGTATGATATAGTACAGTAAGCAAAAAAGTATGAGATGGC
TTGAGATAGTCAAAGATTTTTCTAATAGATCCAAATATGTATCTGGAATTGTCTAGTTCA
CGTTATAATAACACATTTTACCTATCTTCTTCTTAGAAATTATTACGAAACTATATTATA
ACTAACCAAAACATTTTTCATTTGATTATATGCTTAGTGGCAAATGCCATCTTTGATGTC
CGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGC
   
Sequence Name 08-K117141-0022-960-C11C-8409
GenBank Accession LN489903 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117141-0022-960-C11C-8409
CATTTCATTGAATATATCCGGACATCAAAGATGGCATTTGCCACTAAGCATATAATCAAA
TGAAAAATGTTTTGGTTAGTTATAATATAGTTTCGTAATAATTTCTAAGAAGAAGATAGG
TAAAATGTGTTATTATAACGTGAACTAGACAATTCCAGATACATATTTGGATCTATTAGA
AAAATCTTTGACTATCTCAAGCCATCTCATACTTTTTTGCTTACTGTACTATATCATACA
CTTGTAAGTTGTTAATGAATTGGTTATATATATAGCATATATTCATTTTCTTCTAGAAGA
CATGGTTGCTTCTGCTAAATAAAA

Last Updated on 06.12.2018 16:11