Sequences from confirmation amplicon


Confirmation sequences for line 960D01 and hit At5g54030 are shown.
Line ID 960D01
Locus At5g54030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21926763
Primers used for PCR 23rr - 8474, show primer details
   
Sequence Name 40-K116272-0022-960-D01C-23rr
GenBank Accession LN503832 [GenBank]
Sequencing primer gene specific primer, 23rr: 5'-CATTTATAACCTCCATAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116272-0022-960-D01C-23rr
CTCCATCTCCTTCTTTTTACAAGATGAAAAGTATGAGATATGCGATGGTCATGCCGATTT
ATGCTTATGACTCGCGGTAGATTCTCAACACAATCTTTGTGGACCATCAGATTGCATTCA
AGACATACGTAAGGATTGCGGTCGCCTTCCACACCACAAGCATTTACAAGTAAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCG
   
Sequence Name 16-K116271-0022-960-D01C-8409
GenBank Accession LN493581 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116271-0022-960-D01C-8409
GAAGGCGACCGCAATCCTTACGTATGTCTTGAATGCAATCTGATGGTCCACAAAGATTGT
GTTGAGAATCTACCGCGAGTCATAAGCATAAATCGGCATGACCATCGCATATCTCATACT
TTTCATCTTGGTAAAAAAGAAGGAGATTGGGAGTGTGGAGTTTGTCGGAAGATGATTAAT
TGCGTCTATGAGGGTTATAAATGAANAAAAAGAAGGAGTNTTTACCATTTTTTT

Last Updated on 06.12.2018 16:11