Sequences from confirmation amplicon


Confirmation sequences for line 960E03 and hit At1g60095 are shown.
Line ID 960E03
Locus At1g60095 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22160016
Primers used for PCR 10ok - 8474, show primer details
   
Sequence Name 53-K114805-0022-960-E03C-10ok
GenBank Accession LN508239 [GenBank]
Sequencing primer gene specific primer, 10ok: 5'-CCTCCTTTTGCTTCCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K114805-0022-960-E03C-10ok
AACCCGATGATCTTCTTTCCATCCTCAGCTAGTGAAAACGTAGTAGGATTACCAAATATA
GCTTTTTCAAATCCTATGATTTCAGAAATCCTCAAGTTGGTTTTGAATTGAACTCCTTGA
ATCAAATCCGACCCGGGCTTGCAATAACCCTCAACAGATACCAGTTGTTCATCTTTTTTA
TGGTCAATCTCAAACTGCATATAATTTGGTACAAAAAAAAAATTCAAATCATAGTCAATA
GTATCCACATTAAAACTAAACTAAAGAACTTTAGTTAGTATCTTTCAACTAAAGATACCG
TTGGGAGGAGGCCTTGAAAAGTGGGACCATGGAACGATCCAAATTTGGGTTGTCCACTCT
GGATATAGTCAAATCGGAGGTTCCTTATGCATTCTTTACCAAAGCTAACATATTTTTTTG
TTACGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATT
   
Sequence Name 29-K114804-0022-960-E03C-8409
GenBank Accession LN499409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114804-0022-960-E03C-8409
CATTTACAATGAATATATCGTAACAAAAATATATGTCAAGCTTTGGTAAAGAATGCATAA
GGAACATCCGATTCGACTATATCAAGAGTGGACAACCAAAATATGGATCGTTCCATGGTC
CCACTTTTCAAGGCCTCCTCCAAACGGTATCTTTAGTTGAAAGATACTAACTAAAGTTCT
TTAGTTTAGTTTTAATGTGGATACTATTGACTATGATCTGAATTTTTTTTTTGTACCAAA
TTATATGCAGTTTGAGATTGACCATAAAAAAGATGAACAACTTGTATCTGTTGAGGGTTA
TTGCAAGCCCGGGTCGGATTTGATTCAAGGAGTTCAATTCAAAACCAACTTGAGGATTTC
TGAAATCATAGGATTTGAAAAAGCTATATTTGGTAATCCTACTACGTTTTCACTAGCTGA
GGATGGAAAGAAGATCATCGGGTTTCACGGATATTCCATGGCAAACCTAAACTCTCTTGG
AGCATACTTCACTTGGATTTCTCCAACTAAAATGG

Last Updated on 06.12.2018 16:11