Sequences from confirmation amplicon


Confirmation sequences for line 961A08 and hit At1g75410 are shown.
Line ID 961A08
Locus At1g75410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28301283
Primers used for PCR 66by - 8474, show primer details
   
Sequence Name 88-K108479-0022-961-A08C-66by
GenBank Accession LN514874 [GenBank]
Sequencing primer gene specific primer, 66by: 5'-AATCCATTTACCTCAACGAACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K108479-0022-961-A08C-66by
CAGCTTCTTCTTCTCTGCTGCTCTTTCTCCGAGATTGTTTCCGGTGATGTTCGAAACAAC
GAGATGGTATTTATCCCACCAACAAGCGACGTAGCCGTCAACGGAAACGTAACGGTGTCA
AGTAACGATCTAAGCTTTCACGGTGGAGGACTTTCTTTAAGTCTTGGTAATCAGATCCAG
TCAGCTGTCTCTGTTTCTCCGTTTCAGTATCATTACCAGAATCTTTCGAACCAATTGAGT
TACAATAATCTTAATCCTTCTACTATGTCTGATGAGAATGGGAAGAGCTTGAGTGTTCAT
CAGCATCACTCTGATCAAATTTTACCTTCCTCTGTTTACAACAACAATGGTAATAATGGT
GTTGGATTCTACAACAATTACCGTTACGAGACATCAGGGTTTGTGAGTAGTGTACTGAGA
TCTCGTTACCTTAAACCAACACAACAATTGCTTGATGAAGTTGTTAGTGTAAGGAAAGAT
TTGAAATTGGGGAATAAGAAGATGAAGAATGATAAAGGCGAAAAATGAAAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 76-K108478-0022-961-A08C-8409
GenBank Accession LN513413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K108478-0022-961-A08C-8409
GCCATTTTTATTTTTCGCCTTTATCATTCTTCATCTTCTTATTCCCCAATTTCAAATCTT
TCCTTACACTAACAACTTCATCAAGCAATTGTTGTGTTGGTTTAAGGTAACGAGATCTCA
GTACACTACTCACAAACCCTGATGTCTCGTAACGGTAATTGTTGTAGAATCCAACACCAT
TATTACCATTGTTGTTGTAAACAGAGGAAGGTAAAATTTGATCAGAGTGATGCTGATGAA
CACTCAAGCTCTTCCCATTCTCATCAGACATAGTAGAAGGATTAAGATTATTGTAACTCA
ATTGGTTCGAAAGATTCTGGTAATGATACTGAAACGGAGAAACAGAGACAGCTGACTGGA
TCTGATTACCAAGACTTAAAGAAAGTCCTCCACCGTGAAAGCTTAGATCGTTACTTGACA
CCGTTACGTTTCCGTTGACGGCTACGTCGCTTGTTGGTGGGATAAATACCATCTCGTTGT
TTCGAACATCACCGGAAACAATCTCGGAGAAAGATGCAGCAGAGGAAGAAGAAGCTTGTT
GTTGTTGTTGTTGTTGTTCGTTGAGGTAAATGGATT

Last Updated on 06.12.2018 16:11