Sequences from confirmation amplicon


Confirmation sequences for line 962A05 and hit At5g23260 are shown.
Line ID 962A05
Locus At5g23260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7836418
Primers used for PCR 50lx - 8474, show primer details
   
Sequence Name 84-K114129-0022-962-A05C-50lx
GenBank Accession LN514459 [GenBank]
Sequencing primer gene specific primer, 50lx: 5'-ATTACGAAAAAGCTGGATCAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K114129-0022-962-A05C-50lx
TTTGACCATACGTATTAATATGATAAGATACATGAAAATACAAATTAAATACTGCAAGAA
CAAAAATATTGGATCAAAGATTCTTAACTTCTGGGCCAAATGAAAATCCAAAAAACGACT
CATGACACCTGCTACTTCAGAAACTAATGATGAAAACTGTCTGTTCTCAAGCACGAGTCA
TATATCTAGCTCATTTCCTCATTAATTAGATGGATACATACACATGTATGTATAGATCTC
TGAAACCCTAATTCAAGAAAGAAGAAGAAGTTAACCTGTTCTGTTCGGAGCAGAACTCGG
AAAGCTTTCCGGTGGCTGAGAAGACGATGAGACCGATGTGAGCGTCACAGAGAATAGAGA
GCTCACGAGTCTTCTTTATAAGACCAGTTCTTCTCTTGGAGAAGGTCACTTGCCTCGCCG
TCTGATTCTCTATCTTCTTTATCTCTATCTTCCCTCTACCCATCTCTCTCTTCTTCCTCT
TATGAGTATCTACCAACCAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCA
   
Sequence Name 42-K114128-0022-962-A05C-8409
GenBank Accession LN504544 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114128-0022-962-A05C-8409
TTTGGTTGGTAGATACTCATAAGAGGAAGAAGAGAGAGATGGGTAGAGGGAAGATAGAGA
TAAAGAAGATAGAGAATCAGACGGCGAGGCAAGTGACCTTCTCCAAGAGAAGAACTGGTC
TTATAAAGAAGACTCGTGAGCTCTCTATTCTCTGTGACGCTCACATCGGTCTCATCGTCT
TCTCAGCCACCGGAAAGCTTTCCGAGTTCTGCTCCGAACAGAACAGGTTAACTTCTTCTT
CTTTCTTGAATTAGGGTTTCAGAGATCTATACATACATGTGTATGTATCCATCTAATTAA
TGAGGAAATGAGCTAGATATATGACTCGTGCTTGAGAACAGACAGTTTTCATCATTAGTT
TCTGAAGTAGCAGGTGTCATGAGTCGTTTTTTGGATTTTCATTTGGCCCAGAAGTTAAGA
ATCTTTGATCCAATATTTTTGTTCTTGCAGTATTTAATTTGTATTTTCATGTATCTTATC
ATATTTAATACGTATGGGTCAAATTCTATATTTAATTTGTATTTGACATTTCTTGATCCA
GCTTTTTCGTAA

Last Updated on 06.12.2018 16:11