Sequences from confirmation amplicon


Confirmation sequences for line 963C09 and hit At2g31820 are shown.
Line ID 963C09
Locus At2g31820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13531105
Primers used for PCR 92qa - 8474, show primer details
   
Sequence Name 53-K115550-0022-963-C09C-92qa
GenBank Accession LN508251 [GenBank]
Sequencing primer gene specific primer, 92qa: 5'-AAACGTGGAGTTATGGAGAAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K115550-0022-963-C09C-92qa
TTCCGTGGTGGGTTTTTGGAGAACAGAAGAGCTTCCGTGTGGTGATGGAGAGGCAATTGA
GTTTTATAGGTGAGAGGAGGAAGAAGAATGAATCACCAGGGAAAAGAGGAGACTCTTCTC
TTCATATCGCTGCGAGAACCGGGAACTTAAGTAAGGTTAAAGAACTGATTCGAGGTTGTG
GTGATGAGTTGAAAGAGTTGTTGTCAAAGCAAAATCTTGAAGGAGAGACTCCTCTTTATA
CTGCAGCAGAGAATGGGCATTCAATTGTTGTTGAGGAGATGTTGAAGCATATGGACCTTG
AAACTGCTTCCATCGCTGCTAGAAACGGGTTTGATCCATTTCATGTCGCCGCCAAACAAG
GCCATCTCGGTATAAAGATTTATACTTTCTTCATATTTTCTCCATTCTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 26-K115549-0022-963-C09C-8409
GenBank Accession LN498063 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115549-0022-963-C09C-8409
ATTTCATTGAGAATGGAGAAATATGAAGAAATATAAATCTTTATACCGAGATGGCCTTGT
TTGGCGGCGACATGAAATGGATCAAACCCGTTTCTAGCAGCGATGGAAGCAGTTTCAAGG
TCCATATGCTTCAACATCTCCTCAACAACAATTGAATGCCCATTCTCTGCTGCAGTATAA
AGAGGAGTCTCTCCTTCAAGATTTTGCTTTGACAACAACTCTTTCAACTCATCACCACAA
CCTCGAATCAGTTCTTTAACCTTACTTAAGTTCCCGGTTCTCGCAGCGATATGAAGAGAA
GAGTCTCCTCTTTTCCCTGGTGATTCATTCTTCTTCCTCCTCTCACCTATAAAACTCAAT
TGCCTCTCCATCACCACACGGAAGCTCTTCTGCTTCTCCAAAAACCCACCACGGAAACTC
TTCTGCCTCTCCATTGTTGCTCTACCAAAACTCGGTAGCTTCTCCATAACTCCACATT

Last Updated on 06.12.2018 16:11