Sequences from confirmation amplicon


Confirmation sequences for line 963G04 and hit At3g48740 are shown.
Line ID 963G04
Locus At3g48740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18054345
Primers used for PCR 79we - 8474, show primer details
   
Sequence Name 91-K118393-0022-963-G04C-79we
GenBank Accession LN515142 [GenBank]
Sequencing primer gene specific primer, 79we: 5'-CCTATATATACGTGAAGTCCCCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K118393-0022-963-G04C-79we
GAGAAACACTTGCAAACTAAGTGCTATCTCTCTTCTTCTTCCTTCTCCTAACAACTTATA
TACCATGAGTCTCTTCAACACTGAAAACACATGGGCCTTTGTCTTTGGCTTGCTCGGTAT
GATACTAACGTTGTGTTCATTTCTTATGAATATTTTTGATCAAGTTCTGCAACCCTAATA
TCTAAATTCTAGAGATTTAATATCGATTTCTTAATATCATATTTTTGCTATATTCTTGTA
TAATTTTCAGGCAACCTTATCTCCTTTGCCGTGTTCCTATCTCCTGTGTAAGTAATATAT
ATGCAACAAATCAGAATATATATGACTACGTTACATCAATATTCTCCGATATAATTGAGT
AACTAATATTCAGTTTTTTTTTGTAATCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 43-K118392-0022-963-G04C-8409
GenBank Accession LN504999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118392-0022-963-G04C-8409
TGATTACAAAAAAAAACTGAATATTAGTTACTCAATTATATCGGAGAATATTGATGTAAC
GTAGTCATATATATTCTGATTTGTTGCATATATATTACTTACACAGGAGATAGGAACACG
GCAAAGGAGATAAGGTTGCCTGAAAATTATACAAGAATATAGCAAAAATATGATATTAAG
AAATCGATATTAAATCTCTAGAATTTAGATATTAGGGTTGCAGAACTTGATCAAAAATAT
TCATAAGAAATGAACACAACGTTAGTATCATACCGAGCAAGCCAAAGACAAAGGCCCATG
TGTTTTCAGTGTTGAAGAGACTCATGGTATATAAGTTGTTAGGAGAAGGAAGAAGAAGAG
AGATAGCACTTAGTTTTGCAAGTGTTTTCTCTTAATGTAAAAGCTAATGGCACGAGCTTA
GTGTTTCGGGGACTT

Last Updated on 06.12.2018 16:11