Sequences from confirmation amplicon


Confirmation sequences for line 964E08 and hit At1g23260 are shown.
Line ID 964E08
Locus At1g23260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8257343
Primers used for PCR 24sr - 8474, show primer details
   
Sequence Name 52-K116569-0022-964-E08C-24sr
GenBank Accession LN507929 [GenBank]
Sequencing primer gene specific primer, 24sr: 5'-TTAGAGTGGACAAAAAGGAAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K116569-0022-964-E08C-24sr
TTGATGTTTTTTGTTTGGTGATGAACGAATATCAGACTGCATATGAAGGGAAAATCTTTC
AGCTGAAGCTTTTCTGTGGTAAGGAGTACCCTGAAAGTCCACCGACTGTGAGGTTCCAGA
CCCGGATAAACATGGCTTGTGTTAACCCAGAAACTGGAGTGGTAATAACCTTTGACATCA
CTGCTGTTATATATGTGTGTGTGTGTGTGGATATTTGCTTAGTCTGACAAGTTGTTTCGA
AATATGAAATCAGGTTGAACCGAGTCTCTTCCCTATGCTCACTAACTGGCGGCGAGAATA
TACAATGGAGGACATACTGGTTAAGCTGAAAAAAGAAATGATGACTTCGCATAACCGGAA
GTTAGCTCAACCCCCGGAAGGTAATTTCTCTAGTTTGCAAAACCCAAATTCGGTTTATAA
TTACTGTCATGGATTAGTGGGGATGACTTGATAAGATATCACTTTCTGGTTTAAACATCT
TTGATAGATATTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCACA
   
Sequence Name 23-K116568-0022-964-E08C-8409
GenBank Accession LN496711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K116568-0022-964-E08C-8409
CATTTACATTGAATATATCCTGTAATATCTATCAAAGATGTTTAAACCAGAAAGTGATAT
CTTATCAAGTCATCCCCACTAATCCATGACAGTAATTATAAACCGAATTTGGGTTTTGCA
AACTAGAGAAATTACCTTCCGGGGGTTGAGCTAACTTCCGGTTATGCGAAGTCATCATTT
CTTTTTTCAGCTTAACCAGTATGTCCTCCATTGTATATTCTCGCCGCCAGTTAGTGAGCA
TAGGGAAGAGACTCGGTTCAACCTGATTTCATATTTCGAAACAACTTGTCAGACTAAGCA
AATATCCACACACACACACACATATATAACAGCAGTGATGTCAAAGGTTATTACCACTCC
AGTTTCTGGGTTAACACAAGCCATGTTTATCCGGGTCTGGAACCTCACAGTCGGTGGACT
TTCAGGGTACTCCTTACCACAGAAAAGCTTCAGCTGAAAGATTTTCCCTTCATATGCAGT
CTGATATTCGTTCATCACCAAACAAAAAACATCAATCTTCTCAAAATCCAAAAGCACAAA
CCAACAATTTCCTTCCTTTTTGTCCACTCTA

Last Updated on 06.12.2018 16:11