Sequences from confirmation amplicon


Confirmation sequences for line 964F06 and hit At5g63595 are shown.
Line ID 964F06
Locus At5g63595 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25459803
Primers used for PCR NA64 - 8474, show primer details
   
Sequence Name 65-K101672-0022-964-F06C-8409
GenBank Accession LN511359 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K101672-0022-964-F06C-8409
CCATTTACTGCAACCGAGGATCTCTTCCGCTAGCTGCTTTGCATGCTTTGCAAACTCCTC
AGTCACCTCTCTGACCCAAAATCGCAGCGGTTAATGTTTTTAGTTAGCACGTTGTTTAAT
TTGGGTAAATGCATAAATGAAATACGTACATGATTTCCATATATCATAGCACAAATTAAA
CGGTAAATTATAAAAAAAAAAAAAATGTTTGACCCATCTGTATATATATATATATATATA
TATATAAAACCTGTTTTCGGGAGGATTCTTAGGCCAAAATGCATAATTGACTGACAAAAA
TGGATATATATTATGGAAAATATGACCCCTTCTCCCTTGTACATCCCCCCTCTCATATCC
TTGTATCTTTTTGGAGTTCGCGGGTTTGGCCCCGGTTTTTTTTTGGGACTCGGGGAGCTC
AAAAAAGTGTGTATCCCCCTGGGGCACCCCCCGGATTATTTCGGGGGGGACCCCGGGGTT
AACCCCCAAAAAAATTCCCCACTCTTCGCTCGCCTTTGCCATTTTACGCCCCAAGAGCCC
CCCATTGGTGTTTCTTAGGTCCACAACGGGAAATTTTTGTGAAGAGGGGATTTTGTTTTT
TTGAAA
   
Sequence Name 65-K101679-0022-964-F06C-NA64
GenBank Accession LN511360 [GenBank]
Sequencing primer gene specific primer, NA64: 5'-CCGTTGTCTCTACAAAACAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K101679-0022-964-F06C-NA64
TCGACCTAGCAACACCAATGGGGAGCTCGTGGCGCGTAAAGTGGCAAAGGCGAGCGAAGA
GTGGGGAATTTTTCAGGTAGTTAACCACGGGATCCCAACCGAACTAATCCGGAGGTTGCA
CAAGGTGGATACACAGTTCTTTGAGCTCCCAGAGTCCAAGAAAGAAGCCGTGGCCAAACC
AGCGAACTCCAAAGAGATACAAGGATATGAGATGGATGATGTACAAGGTAGAAGGAGTCA
TATCTTCCATAATCTATATCCATCATCGTCAGTCAATTATGCATTCTGGCCTAAGAATCC
TCCTGAATACAGGTTATATATATATATATATATATATATATACAGATGGATCAAACATTT
TTTTTTTTTTTAAAATTTCCCGTTTAATTTGGGCTATGATATAGGGAAATCATGTACGTT
TTTCTTTTTTGCTTTTCCCCAAATAAAACAACGTGCTAACAAAAAAAATTACCCCCTGCT
ATTTTGGGTCAAAAAGGTGACTGAGGATTTTGCAAAGCATGCAAAGCCTCTGGGGAAAAA
AACCCCCGGTTTGCATGAAAAGGGCTTCATGTCCGGGAAATCTCCGGGGATCCCCAATGA
GTATGAGGGTCAATTTGGAGAAAAAAAAAGAGTATTTCCCATTTTTTTTTCAATTCAAAA
AAGGAAAT

Last Updated on 06.12.2018 16:11