Sequences from confirmation amplicon


Confirmation sequences for line 965C01 and hit At1g15030 are shown.
Line ID 965C01
Locus At1g15030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5178991
Primers used for PCR 29vd - 8474, show primer details
   
Sequence Name 24-K117874-0022-965-C01C-29vd
GenBank Accession LN497172 [GenBank]
Sequencing primer gene specific primer, 29vd: 5'-ACATACGCATGTCACAAAGAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117874-0022-965-C01C-29vd
AATCACAGCTTCTTAGCGTCTTCAGCTCGGGAAATCTAGAGGCAAGGTCAGACATCTGCA
AAGGTAACAATTGAATAAGACAGCTATAGGAATTGCATTTTTTTAGATCATAAGTGGTGT
ATTCAAATTCAAGAATAAACAAGTCGCTGGACAGTAATTGAACTTTATACAGTTAGTTGA
TAGTCCTTAATGGATGCTTTGGAACGAACAGAAGAGTTGTTGCATTTGACTAATAGCATT
ACTTTCATAAATAAATCTTGGATGTTACTTCATGAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCCACCGATAA
TTAAAAAGTTCGTGAAAGGGTTAAGGCTCAGCTTGGTCGTGTTGAGGAAGAGACTCGTCG
TCTTGCTAGTATCCGCGAGGTTTGTTTTCATTTTTTGCTTTTTAAAGTCCAAAACTTTAA
GATCTGAGAGATTTTGTTTTGAGCTTGGTCTGAAAATTACACTCATGCAAGTTGACTACT
TTCTGTGTGTTTGTCTTTTTTCTGAATTGAATTTTGATACTGTTTATGTGGTTTTAGAAT
ATTAGGGTTTTGAAA
   
Sequence Name 12-K117873-0022-965-C01C-8409
GenBank Accession LN491775 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117873-0022-965-C01C-8409
TCTTCTGTTCGTTCCAAGCATCCCTTAAGGACTATCAACTAGCTGTATAAAGTTCAATTA
CTGATCCAGCGACTCGTTTTATTCTTGAATTTGAGTACGCCACTTATGATCTAAAAAAAT
GCAATTCCTATCGCTGTCTGACTCAATTGATACCTTTGCAGATGTCTGACCTTGCCTC

Last Updated on 06.12.2018 16:11