Sequences from confirmation amplicon


Confirmation sequences for line 965D10 and hit At1g04950 are shown.
Line ID 965D10
Locus At1g04950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1404559
Primers used for PCR 31wu - 8474, show primer details
   
Sequence Name 54-K118874-0022-965-D10C-31wu
GenBank Accession LN508639 [GenBank]
Sequencing primer gene specific primer, 31wu: 5'-GGATAGCTGTGCTAAAGTTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118874-0022-965-D10C-31wu
CGTTAGACGATAAAGTCCTAATTTCCCAATAAAATGAATAAGCAAACCCGGAAAAATGGT
ATGGGCAAATCAAATTGTATCTCAGATTCTGAAAAGTCCCTAGATTGAAGGACAAGCTGG
GTAACATTATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGTCAATTATT
   
Sequence Name 14-K118873-0022-965-D10C-8409
GenBank Accession LN492736 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118873-0022-965-D10C-8409
ATTGAAATAATGTTACCCAGCTTGTCCTTCAATCTAGGGACTTTTCAGAATCTGAGATAC
AATTTGATTTGCCCATACCATTTTTCCGGGTTTGCTTATTCATTTTATTGGGAAATTAGG
ACTTTTATCGTCTTAAACGGCTACTCCAGTGAATCAGATACCCCTGCTACCAAAAACTTT
AGCACAGCTATCC

Last Updated on 06.12.2018 16:11