Sequences from confirmation amplicon


Confirmation sequences for line 965E04 and hit At1g16110 are shown.
Line ID 965E04
Locus At1g16110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5518689
Primers used for PCR 74lx - 8474, show primer details
   
Sequence Name 31-K114086-0022-965-E04C-74lx
GenBank Accession LN500253 [GenBank]
Sequencing primer gene specific primer, 74lx: 5'-ACCCTTCTTTCCCTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114086-0022-965-E04C-74lx
ATACCTGAGGACCTCCACGGTATTCTCGCCTGGCAGCATTTGTAACCGTCACATTTCGAG
TTTGTTACTTCTTCACTACTCTTGCTATCTTCACAGCTCGATTCACAACCCAAGATCTCT
GATTCTATATCCTTCATCAAAGCCTTTGTACCACAACCAACCATCACGAGGCAGTTCTCA
TCAGTGAGGAAATATGGACTTCCCCTGCCTGTGACGTTCAAATTCGGCAATGACATTTCG
GATACTTGACTGCTACTACTACTACTAGAGCAACCCAACGATGTCACTGGACCTTTGATG
TGAACTACTCCATATAGTCAATATGCATGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGGTTATT
   
Sequence Name 55-K114085-0022-965-E04C-8409
GenBank Accession LN508877 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114085-0022-965-E04C-8409
TTACATTGAATATATCCATGCATATTGACTATATGGAGTAGTTCACATCAAAGGTCCAGT
GACATCGTTGGGTTGCTCTAGTAGTAGTAGTAGCAGTCAAGTATCCGAAATGTCATTGCC
GAATTTGAACGTCACAGGCAGGGGAAGTCCATATTTCCTCACTGATGAGAACTGCCTCGT
GATGGTTGGTTGTGGTACAAAGGCTTTGATGAAGGATATAGAATCAGAGATCTTGGGTTG
TGAATCGAGCTGTGAAGATAGCAAGAGTAGTGAAGAAGTAACAAACTCGAAATGTGACGG
TTACAAATGCTGCCAGGCGAGAATACCGTTGGAGCGTCNCAGGTTATTGGTATCAATATA
GAAAACACTAGTGCCACAAGAGGGAAAGAAGGG

Last Updated on 06.12.2018 16:11