Sequences from confirmation amplicon


Confirmation sequences for line 965G04 and hit At3g59340 are shown.
Line ID 965G04
Locus At3g59340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21929248
Primers used for PCR 40lu - 8474, show primer details
   
Sequence Name 71-K114180-0022-965-G04C-40lu
GenBank Accession LN512599 [GenBank]
Sequencing primer gene specific primer, 40lu: 5'-ACATCCAGGCATATTCATACATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114180-0022-965-G04C-40lu
TAGTCAACATTTTCTTGTCTTTGGCAAAAAAAATTCCCTTTGTTTTTCCCGGAAAAAATA
ATCTATAATATCTCATATCCCTTTGGTTAGGGTTCCGGTGGAAGCAACTATAACGATGTG
TCTGGAAGTGATTGACCTTCCTCCTCATCCAACAGTTTCTTCCTCTGCTCCTCTCTTTCT
TCTTCCTCATCTTTCTCCTTCCTGATAACATATGGAAAAAACGCAACATGGATTGTGTTA
CTACTTTAAGCCATGATTGTAGAACAGATATTGGAGTAAGGGAAAGAGTGTACATTGAGT
ATATGATTAGTCCAATAGCTGTTGTAGCAAATGCCAAGTAGTAGAGCCAATCAACCTGAC
ATTAAGTTACATACATGATTAATTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 35-K114177-0022-965-G04C-8409
GenBank Accession LN501917 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114177-0022-965-G04C-8409
ATTGAATAATCCTAATTATCATGTATGTAACTTAATGTCAGGTTGATTGGCTCTACTACT
TGGCATTTGCTACAACAGCTATTGGACTAATCATATACTCAATGTACACTCTTTCCCTTA
CTCCAATATCTGTTCTACAATCATGGCTTAAAGTAGTAACACAATCCATGTTGCGTTTTT
TCCATATGTTATCAGGAAGGAGAAAGATGAGGAAGAAGAAAGAGAGGAGCAGAGGAAGAA
ACTGTTGGATGAGGAGGAAGGTCAATCACTTCCAGACACATCGTTATAGTTGCTTCCACC
GGAACCCTAACCAAAGGGATATGAGATATTATAGATTATTTTTTCCGGGAAAAACAAAGG
GAATTTTTTTTGCCTAAAGACAAGAAAATGTTTGACTAATGGCTTTGTCTGTCTGATTGT
TTTGAGTAATAGTATGATGTATGAATATCCCTGGATG

Last Updated on 06.12.2018 16:11