Sequences from confirmation amplicon


Confirmation sequences for line 967C09 and hit At3g54060 are shown.
Line ID 967C09
Locus At3g54060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20018837
Primers used for PCR 20my - 8474, show primer details
   
Sequence Name 45-K114505-0022-967-C09C-20my
GenBank Accession LN505616 [GenBank]
Sequencing primer gene specific primer, 20my: 5'-TGACACAGATACATCAGAGGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114505-0022-967-C09C-20my
TGGAGAGTACTTGGCTTGAGTCATGTACTAAACATAAGCCATTTAATTTCTATTGTAAGC
CAGTTTCTGACTACATGGTATATGCAAATAGATGCATAATGGTGAAAGTCCTATTTTGTT
GTCAATATCAATATAGAGAAGAAATCAGCTGTTAAAACTATAGACCAAGAAACCAAGTTT
AATTAAAACTATAGACCAAAAAACCAAGTTTAATTTATACACCTACATGTAATACTAGTT
AATTATAAGCTACGTTATGGGATAGCCTTTTCATATTTCACAGATCATTACAAGAATAAG
CACTAGCAGCATAGTAAGGAGAAAACAGATATATGATGGATCATAGCAGTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 20-K114504-0022-967-C09C-8409
GenBank Accession LN495313 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114504-0022-967-C09C-8409
CTATGATCCATCATATATCTGTTTTCTCCTTACTATGCTGCTAGTGCTTATTCTTGTAAT
GATCTGTGAAATATGAAAAGGCTATCCCATAACGTAGCTTATAATTAACTAGTATTACAT
GTAGGTGTATAAATTAAACTTGGTTTTTTGGTCTATAGTTTTAATTAAACTTGGTTTCTT
GGTCTATAGTTTTAACAGCTGATTTCTTCTCTATATTGATATTGACAACAAAATAGGACT
TTCACCATTATGCATCTATTTGCATATACCATGTAGTCAGAAACTGGCTTACAATAGAAA
TTAAATGGCTTATGTTTAGTACATGACTCAAGCCAAGTACTTCTCCAGCTTCTCTACTTC
CTCTGTGCTTCCAATGTATAGAGGAACCCTCTGATGTATCTGTGTC

Last Updated on 06.12.2018 16:11