Sequences from confirmation amplicon


Confirmation sequences for line 967G07 and hit At1g52280 are shown.
Line ID 967G07
Locus At1g52280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19468026
Primers used for PCR 24rx - 8474, show primer details
   
Sequence Name 43-K116272-0022-967-G07C-24rx
GenBank Accession LN504965 [GenBank]
Sequencing primer gene specific primer, 24rx: 5'-GTATGCTATGATTTTGGGGACACT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K116272-0022-967-G07C-24rx
CAAAGTCAACTATTTTTATTTACACATTTAGTTTAAGAGTTAATAATATTTACACATCGC
CCCAATCAGATACGCTTAACCCGTATTTATTATTTTAACTTTTTTTGTATTGGTAAAAAC
GCTGAGAGACGATCGATCTTCAAATTCTCATCGCTTTCAGAGACAAGATCCCACAAAAAC
CGTCCAATGTATCCTTCAAATTCAAACCCCTTCTAGAATCGAAATTAAGTTGGATTTTGG
TATCAGTATAGAGTCCAAAAGAAAAAGGAACCCAATTCATTACAATTTGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 19-K116271-0022-967-G07C-8409
GenBank Accession LN494914 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116271-0022-967-G07C-8409
ATCGGGTCCCTTTTTCTTTCGGACTCCTATACCGATACCAAAATCCAACTTAATTTCGAT
TCCTAGAAGGGGTTTGAATTTGAAGGATACATTGGACGGTTTTTGTGGGATCTTGTCTCT
GAAAGCCGATGAGAATTTGAAGATCGATCGTCTCTCAGCGTTTTTACCAATACAAAAAAA
GTTAAATAATAAATACGGGTTAAGCGTATCTGATTGGGGCAATGTGTAAATATTATTAAC
TCTTAAACTAAATGTGTAAATAAAAATAGTTGACTTTGTCAAATATAGATAACACAACTG
AGAAAATCAATTTCCTCTACCGGTAAGCAAATCATAAGAAAACTAGGAAGTGTCCCC

Last Updated on 06.12.2018 16:11