Sequences from confirmation amplicon


Confirmation sequences for line 968A09 and hit At1g61980 are shown.
Line ID 968A09
Locus At1g61980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22908210
Primers used for PCR 63tv - 8474, show primer details
   
Sequence Name 20-K117142-0022-968-A09C-63tv
GenBank Accession LN495385 [GenBank]
Sequencing primer gene specific primer, 63tv: 5'-AGTGATTTCTCAGCATCAGCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117142-0022-968-A09C-63tv
CAGTGAACCATGACTTCTCAAAAGATTCAGAACAGAGTCAGGGTTGTCCTTGTCCTCGAA
GCTAACCTTCCTTGAGACCGATTCAGCGAGCTTTTTGGGTAAACCCAATGAATCAACGAG
GTAAGAGACTGTAAAGCTCTTACCTTTTCGACCAACTCTAGGACTCAAACGCGCAGCAGA
AGCAGAGGAGAAGGAATTGGACAAAGGAGACGCCTTTTGCACTAAAACTCTCAAATGACG
CCAATGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAACGTTATTAT
   
Sequence Name 10-K117141-0022-968-A09C-8409
GenBank Accession LN490836 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117141-0022-968-A09C-8409
TATATCCTGTCCATTGGCGTCATTTGAGAGTTTTAGTGCAAAAGGCGTCTCCTTTGTCCA
ATTCCTTCTCCTCTGCTTCTGCTGCGCGTTTGAGTCCTAGAGTTGGTCGAAAAGGTAAGA
GCTTTACAGTCTCTTACCTCGTTGATTCATTGGGTTTACCCAAAAAGCTCGCTGAATCGG
TCTCAAGGAAGGTTAGCTTCGAGGACAAGGACAACCCTGACTCTGTTCTGAATCTTTTGA
GAAGTCATGGGTTCACTGATTCTCAGATCTCCACCATCGTTACGGATTATCCACAATTGC
TTGTAGCTGATGCTAGNAAATCACT

Last Updated on 06.12.2018 16:11