Sequences from confirmation amplicon


Confirmation sequences for line 971D04 and hit At1g52430 are shown.
Line ID 971D04
Locus At1g52430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19533254
Primers used for PCR 38pf - 8474, show primer details
   
Sequence Name 77-K115259-0022-971-D04C-38pf
GenBank Accession LN513589 [GenBank]
Sequencing primer gene specific primer, 38pf: 5'-GGTTGGTTATATATAGACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K115259-0022-971-D04C-38pf
TTTTTTAATGGTTTCATATCTCTTCTCATCTAGAATGAAATAATGAATATGGAACGCAAA
GCCAAGGCAGATGCAGTAGCAGCAGATCTTGCACTCGAGGAGGAGAAGAAGGCACAGTCA
AAGAAGAAGAATGATAAAATAAACAAGGTCTTTCAACTAGTAATATTCTGTGAACAAGTC
AATGACGTTTTCCTATCTTATTTTGATAACATATCTTCCATTTTGACTTCGTTTCCTTAC
TTTTAGGATTCCACTTCTCTGCTTTGTGTATACCTGTAATGTCACATTGTATTAATGAGT
GATCTTGATGGTGCAGCAGAGAGCTTCAATGAGCAAGTTTAGTCCCCTTGATCAGACTGT
TGAACAGTAAGTACCTTAAGAGTCATTTTCTTATGTTAATTAATTGTTTTCGGGTTTTCT
CTGAGTTTTTACATGTACAGTAAACCTCCTGTCAACCTTGAACTAAAAACGGTGGAAGAA
GATTCTATGGAACCAGAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATATCCGCA
   
Sequence Name 37-K115258-0022-971-D04C-8409
GenBank Accession LN502727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115258-0022-971-D04C-8409
ATTTCTGGTTCATAGAATCTTCTTCCCCGNTTTTTAGTTCAAGGTTGACAGGAGGTTTAC
TGTACATGTAAAAACTCAGAGAAAACCCGAAAACAATTAATTAACATAAGAAAATGACTC
TTAAGGTACTTACTGTTCAACAGTCTGATCAAGGGGACTAAACTTGCTCATTGAAGCTCT
CTGCTGCACCATCAAGATCACTCATTAATACAATGTGACATTACAGGTATACACAAAGCA
GAGAAGTGGAATCCTAAAAGTAAGGAAACGAAGTCAAAATGGAAGATATGTTATCAAAAT
AAGATAGGAAAACGTCATTGACTTGTTCACAGAATATTACTAGTTGAAAGACCTTGTTTA
TTTTATCATTCTTCTTCTTTGACTGTGCCTTCTTCTCCTCCTCGAGTGCAAGATCTGCTG
CTACTGCATCTGCCTTGGCTTTGCGTTCCATATTCATTATTTCATTCTAGATGAGAAGAG
ATATGAAACCATATAAAAAAACAATATAAAGGCCAAGTCAGACAGAAAATGAAAGCTGTC
TATATTAAACCAAC

Last Updated on 06.12.2018 16:11