Sequences from confirmation amplicon


Confirmation sequences for line 972C04 and hit At5g15160 are shown.
Line ID 972C04
Locus At5g15160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4921859
Primers used for PCR 31lq - 8474, show primer details
   
Sequence Name 32-K114086-0022-972-C04C-31lq
GenBank Accession LN500671 [GenBank]
Sequencing primer gene specific primer, 31lq: 5'-ATGTACCAAGGTTTTGGCATAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114086-0022-972-C04C-31lq
TATTAGGGTAGCTATTATGTTTATAGAGAATCACGCATATACAAGTATACAACATGTTAG
AATAGTGCATTATAGTCATAGAAGTACGTGTGGGAGACTGCATTTTGAAGTTTCTAAAAT
TACTTTTGACCATTAACCGGACTTTGAGGAGAGGGGAACATATTTTCCCTAACTTCAGCA
TTAAGCTTCTAGTTAATTTTACTTTTATTATTGATTAACTACATTGAATGTGAGACGACT
ATATTGCTTCACGGACCATCAATTCAATACATTTATGTACAGCACCGACTAATTTTTATA
TTTGGTTTTCTTAGTTTCTATATAACAAGAAAAGTACGAACTCTAGAAAAATAAGAAAAA
GCTGGTTTCTATAAATGGAAGCATCGTTTTCTTATAGTACACACAACCAATCAGATTTGT
TCTCAATTTCAAAACAATGTGTTGGTTAAGTTATGAGCATTTTTATTTTTTTGATAAAGT
AAATGGCTTTAGCTTAAGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 56-K114085-0022-972-C04C-8409
GenBank Accession LN509173 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114085-0022-972-C04C-8409
GCCATTTACATTGACTTAAGCTAAAGCCATTTACTTTATCAAAAAAATAAAAATGCTCAT
AACTTAACCAACACATTGTTTTGAAATTGAGAACAAATCTGATTGGTTGTGTGTACTATA
AGAAAACGATGCTTCCATTTATAGAAACCAGCTTTTTCTTATTTTTCTAGAGTTCGTACT
TTTCTTGTTATATAGAAACTAAGAAAACCAAATATAAAAATTAGTCGGTGCTGTACATAA
ATGTATTGAATTGATGGTCCGTGAAGCAATATAGTCGTCTCACATTCAATGTAGTTAATC
AATAATAAAAGTAAAATTAACTAGAAGCTTAATGCTGAAGTTAGGGAAAATATGTTCCCC
TCTCCTCAAAGTCCGGTTAATGGTCAAAAGTAATTTTAGAAACTTCAAAATGCAGTCTCC
CACACGTACTTCTATGACTATAATGCACTATTCTAACATGTTGTATACTTGTATATGCGT
GATTCTCTATTAAACATAATAGCTAACCCTAAATAAATATCATCAAAGTAAAACTTATGT
GACTATGCCAAAACCNTGGTACATAAGTTTTACTTTGATGATATTTATTTAGGGTTAGCT
ATTATGTTTAATAGAGAATCACGCATATACAAGTATACAACATGTTAGAATAGTGCATTA
TAGTCATAGAAGTACGTGTGGGAGACTGCATTTTGAAGTTTCTAAAATTACTTTTGACCA
TTAACCGGACTTTGAGGAGAGGGGAACATTTTTTCCCTAACTTCAGCATTAAGCTT

Last Updated on 06.12.2018 16:11