Sequences from confirmation amplicon


Confirmation sequences for line 972F08 and hit At2g47485 are shown.
Line ID 972F08
Locus At2g47485 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19486173
Primers used for PCR 32wm - 8474, show primer details
   
Sequence Name 57-K118874-0022-972-F08C-32wm
GenBank Accession LN509558 [GenBank]
Sequencing primer gene specific primer, 32wm: 5'-TGCTTCATAAATTTGAGCATTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118874-0022-972-F08C-32wm
ATTCGTACGTAGCAATAATTTAATGTTTTCCCCATGAACGATTAATAATTTCGGAATGAT
TAAAAAAGATACTAATATATATATATATATATATATATATATATATATACTTTCACCCCC
ACAAAAAAAAACAGATAATATATACCACTATCGCGAACATTGCGTGAGCGAGTGCGTATC
GCCGCGCGCTCTTTAAAAAGAAACCGAGAGTGTCTCCCTCTCTCTCACTACACTCCCCAG
TGCGCACACTCTCTCTCTCTCTGGGGATCTCTAGACACCCACACACAGAGAGAGCAGAGA
GCGAGAGGGAGAGAGGGTGGGGGGGGGGGTAAAGAGGACACCAGGGGCGAGGGGGCTCCG
CCAAGAGGGCGCGCGCGGGCGGGAGAAATGTAGAGCGGGTCAAAGGGGCCCCCACACGGA
AGAGAGGGTGGGGGGGGATCAAGAGAGCGCCCACGCGAGGGATCGAGAAAGGGGGCGCTC
CCCAGTGAGTAAGGGGGGCGAGATATACTCTTGTGTGAGGGGCCGCGTGCGGGGGAAATC
TACGGGGATCCGCAGTGAGTGTGGGGGTCTATAGGGAGAAAAAGAGAGAGTATTCCCCTT
TTTTTTCTCTCCACAAAAGTGTGGATGCCC
   
Sequence Name 17-K118873-0022-972-F08C-8409
GenBank Accession LN494078 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118873-0022-972-F08C-8409
TTTCATTGAATATATCCTGCTCCATTTACTCATTGCTGAGCAGCCTTTTTCAAGATCCTT
AGCTTCGGCACTATCTTGATCCTCCAAAACCGTCTCTTCCGTGTGGGATCCATTTTGACC
CGCTTTACATTTCTCCGGCCCGAGCTCGCCTTCTTGGCAGAACCATCTAGCCTTTGGTAT
CCTCTTAACCTCCGCCAATACCTTATCTCCATCACGATCTCTGCTCACTATGCGTATTGG
TGTCTAGAGATCCAGAGAGAGAGAGAGAATGTGAACAGTGGAGAATGTAGTGAGAGAGAC
GGAGACACTCTAGGTTTCTTTATAAAGAGCACGGATCGATATGCATTCGATAACGTAATG
TTCGATATAGTAGTATATATTATCTGTTTTTTTTTGTTGTTGTTAAAGTATATATATATA
TATATATATATATATATATATATATGTATCTTTTTTACTCTCCCAAAAATTATTACTCGC
GCGTGTGAGAAAACATTAATTTATTGCTACGTAAAATTTTCTCCCCCCACAACAACTATA
TGATTATCTCCAAAGGGGGAGAGTCTGTGCT

Last Updated on 06.12.2018 16:11