Sequences from confirmation amplicon


Confirmation sequences for line 973A04 and hit At3g23270 are shown.
Line ID 973A04
Locus At3g23270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8317434
Primers used for PCR 75zj - 8474, show primer details
   
Sequence Name 03-K127721-0022-973-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K127721-0022-973-A04C-8409
TGTCACTATCATGTATCTGAATAACAAAAATGAATCAATGCATTATAAATTTGACTACAT
ATAAGATATATAACATAATGTATAGAAATTATGAAGAAAACATACCTCAGGGATTTCACT
TCTGGCGCGTCGATTACGACTTTTTTCAATCAAAAATTTAAGGGCAGCAAACCACACCTC
TGTCTCAGCTTTGTCCTTACAAATCTGTTCAAAAAGAAAAGAAAAAAAGAACATATGTAA
TAAGTGAATGTTCAATTTTACAATCTAAGTTGAAATGTTCTCTTTACTAACCAGATCAAG
TGACCTTTCTCTGTTGTTATATAGAAGTGAAAATGATAAGTGATCTTTCTCTGGACGTAG
AAATCTCTTAAAAACAGCCTATTCATGAAAAATGGTTAGAAACCGAAAAAAAGAACTTGC
TTTTAAGTAAAAGATTCGGTTAAACTCACAGTTCTTTGTCCCGGGACAATACGAGAAACT
TCAAATAACTTCAAACCTTTTTCTTCTCCACGCGAGAACCAAATCAACGTTTTTTCATCC
TATATTAATAAACATGGAGTGTCATCATATACTATGAGCGCAATGTAATAGTAAGCAC
   
Sequence Name 27-K127720-0022-973-A04C-75zj
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 75zj: 5'-CGTGCTTACTATTACATTGCGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K127720-0022-973-A04C-75zj
GGATGAAAAACGTTGATTTGGTTCTCGCGTGGAGAAGAAAAAGGTTTGAAGTTATTTGAA
GTTTCTCGTATTGTCCCGGGACAAAGAACTGTGAGTTTAACCGAATCTTTTACTTAAAAG
CAAGTTCTTTTTTTCGGTTTCTAACCATTTTTCATGAATAGGCTGTTTTTAAGAGATTTC
TACGTCCAGAGAAAGATCACTTATCATTTTCACTTCTATATAACAACAGAGAAAGGTCAC
TTGATCTGGTTAGTAAAGAGAACATTTCAACTTAGATTGTAAAATTGAACATTCACTTAT
TACATATGTTCTTTTTTTCTTTTCTTTTTGAACAGATTTGTAAGGACAAAGCTGAGACAG
AGGTGTGGTTTGCTGCCCTTAAATTTTTGATTGAAAAAAGTCGTAATCGACGCGCCAGAA
GTGAAATCCCTGAGGTATGTTTTCTTCATAATTTCTATACATTATGTTATATATCTTATA
TGTAGTCAAATTTATAATGCATTGATTCATTTTTGTTATTCAGATACATGATAGTGACAC
TTTCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11