Sequences from confirmation amplicon


Confirmation sequences for line 973C04 and hit At3g21760 are shown.
Line ID 973C04
Locus At3g21760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7667635
Primers used for PCR 22hq - 8474, show primer details
   
Sequence Name 50-K112810-0022-973-C04C-22hq
GenBank Accession LN507223 [GenBank]
Sequencing primer gene specific primer, 22hq: 5'-TACAATCTTTCCTATCTCTGCCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K112810-0022-973-C04C-22hq
CTCAGATTCGTAATTCTTCGGGAGGTCCTATCGATCCTTTTGGTTGAGCACGACGAAGAG
ACCAGACAAAGCGGTGACCACTTCGCTCAAGCGCGATTGCGATTTCTTTAGCTTGGCCCT
CACGGAAACCTCCCATGCTTCCGAAACAGAGGAAAACAACGGATTTACGTGGCTGCTCGT
CTAGCCACCGTAGGATCTCCGATTGCTTATCGTCAGATGAATTTGGACCGTTGATTTTAA
GATTCATAACCGGTCCAACTGTGTACACCGTAGGCAGAGGACTATCTACGCCGGAGAAAA
ACTTCATAGCTTGAGGCTCAAGCTCAGCGAATGTATTTACCAAAATACCTTTAGTTTCTC
GGAATCTTCTGGTTTGTCTAAACATAACCGGTAACCACTCCTTGGTTAATAGAACCGAGG
GGAAACACTTAACCGGTAAAGGACGAGTCAAACAAGGAACTTCCAGCTCAGTAGTGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTA
   
Sequence Name 25-K112809-0022-973-C04C-8409
GenBank Accession LN497522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112809-0022-973-C04C-8409
GGAGTTCCTTGTTTGATCGTCCTTTACCGGTTAAGTGTTTCCCCTCGGTTCTATTAACCA
AGGAGTGGTTACCGGTTATGTTTAGACAAACCAGAAGATTCCGAGAAACTAAAGGTATTT
TGGTAAATACATTCGCTGAGCTTGAGCCTCAAGCTATGAAGTTTTTCTCCGGCGTAGATA
GTCCTCTGCCTACGGTGTACACAGTTGGACCGGTTATGAATCTTAAAATCAACGGTCCAA
ATTCATCTGACGATAAGCAATCGGAGATCCTACGGTGGCTAGACGAGCAGCCACGTAAAT
CCGTTGTTTTCCTCTGTTTCGGAAGCATGGGAGGTTTCCGTGAGGGCCAAGCTAAAGAAA
TCGCAATCGCGCTTGAGCGAAGTGGTCACCGCTTTGTCTGGTCTCTTCGTCGTGCTCAAC
CAAAAGGATCGATAGGACCTCCCGAAGAATTTACGAATCTTGAGGAAATTCTCCCGGAAG
GATTCTTGGAACGGACGGCAGAGATAGAAAAGATTGTA

Last Updated on 06.12.2018 16:11