Sequences from confirmation amplicon


Confirmation sequences for line 973C07 and hit At1g17260 are shown.
Line ID 973C07
Locus At1g17260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5907541
Primers used for PCR 23hq - 8474, show primer details
   
Sequence Name 32-K112877-0022-973-C07C-8409
GenBank Accession LN500654 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K112877-0022-973-C07C-8409
CATTTACGTAAGTTACATCAACTTACTTGAACTTTCTCTTCTTGACATTGTTTCATGCAG
CTTGGTTTTACACTTTTAGCGTTGATATGGGAATACGACTTCCCACCTTTCATGGTTCTG
ATAATCGCAATACTCAATGACGGTAATCTCTTAATCAATAGCTTCATATTGATGTAACCT
AATTTTTATCACTTTCAAACGCTATGTTTATCTCCTACGCATCAGGGACTATCATGACTA
TTTCTAAAGATCGAGTTAGGCCATCTCCTACACCCGAGAGTTGGAAGCTCAACCAGATAT
TTGCGACAGGAATTGTCATTGGAACATATCTAGCATTGGTCACCGTCCTGTTTTACTGGA
TCATTGTTTCTACCACCTTCTTCGAGGTATCTTCCTATCACTCAAAGTAAGTTAGATTTT
GCTTACCGAGAAGAATGCATACCGGTGACCAATCTTTAATGGCTGCAGAAACACTTCCAT
GTAAAATCAATTGNCAACAAC
   
Sequence Name 69-K112878-0022-973-C07C-23hq
GenBank Accession LN512236 [GenBank]
Sequencing primer gene specific primer, 23hq: 5'-TGTTGTTGGCAATTGATTTTACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K112878-0022-973-C07C-23hq
GCATTCTTCTCGGTAAGCAAATCTAACTTACTTTGAGTGATAGGAAGATACCTCGAAGAA
GGTGGTAGAAACAATGATCCAGTAAAACAGGACGGTGACCAATGCTAGATATGTTCCAAT
GACAATTCCTGTCGCAAATATCTGGTTGAGCTTCCAACTCTCGGGTGTAGGAGATGGCCT
AACTCGATCTTTAGAAATAGTCATGATAGTCCCTGATGCGTAGGAGATAAACATAGCGTT
TGAAAGTGATAAAAATTAGGTTACATCAATATGAAGCTATTGATTAAGAGATTACCGTCA
TTGAGTATTGCGATTATCAGAACCATGAAAGGTGGGAAGTCGTATTCCCATATCAACGCT
AAAAGTGTAAAACCAAGCTGCATGAAACAATGTCAAGAAGAGAAAGTTCAAGTAAGTTGA
TGTAACTTACTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCACGGTTATTA
Primers used for PCR 44ve - 3144, show primer details
   
Sequence Name 33-K117497-0022-973-C07C-44ve
GenBank Accession LN501164 [GenBank]
Sequencing primer gene specific primer, 44ve: 5'-TTTTACAAGAAATGAAGCATGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117497-0022-973-C07C-44ve
CTCTCAAAAAGCTGACATCGGAATAGCTGTCGCAGATGCAACAGATGCTGCAAGAAGTTC
TGCTGACATAGTACTAACTGATCCCGGCTTAAGTGTAATTATCAGTGCTGTCTTGACCAG
CAGAGCCATTTTCCAGCGGATGAGGAACTATACAGTAAGTACTAAAGTTATAGTTATACT
ACTATCATCTGATCCCTGATAGCTAAAGAAATGAAAAATCATATAATATAGGATATGGAT
TTCAAGTAGCCTGATGACATTCTCAACTAGCTTATGTTTTTCTCTGAATTACCGACTAAT
CAGTACGAATCTGCAGGTATATGCAGTCTCTATCACCATACGCATAGTGGTAAGGAGCTT
CATGATACATGATCTAACTCAATGACGGGAAATCTACATGGATCAAACACTGATAGTTTA
AACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATG
AACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTA
CGTCAGTGGAGAT
   
Sequence Name 01-K117496-0022-973-C07C-3144
GenBank Accession LN486754 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117496-0022-973-C07C-3144
CAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGA
TCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATCCATGTAGATTTCC
CGTCATTGAGTTAGATCATGTATCATGAAGCTCCTTACCACTATGCGTATGGTGATAGAG
ACTGCATATACCTGCAGATTCGTACTGATTAGTCGGTAATTCAGAGAAAAACATAAGCTA
GTTGAGAATGTCATCAGGCTACTTGAAATCCATATCCTATATTATATGATTTTTCATTTC
TTTAGCTATCAGGGATCAGATGATAGTAGTATAACTATAACTTTAGTACTTACTGTATAG
TTCCTCATCCGCTGGAAAATGGCTCTGCTGGTCAAGACAGCACTGATAATTACACTTAAG
CCGGGATCAGTTAGTACTATGTCAGCAGAACTTCTTGCAGCATCTGTTGCATCTGCGACA
GCTATTCCGATGTCAGCTTTTTTGAGAGCAGGAGCATCATTCACACCATCTCCGGTCATT
CCAACCACATGCTTCATCCTTGGTAAAA

Last Updated on 06.12.2018 16:11