Sequences from confirmation amplicon


Confirmation sequences for line 973D03 and hit At3g45400 are shown.
Line ID 973D03
Locus At3g45400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16652603
Primers used for PCR 24hq - 8474, show primer details
   
Sequence Name 37-K112877-0022-973-D03C-8409
GenBank Accession LN502676 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112877-0022-973-D03C-8409
ATCCTGATCTCTACCACCACTCCTCTTCCACTCCTCTTGAGCAGTCAAAAACGTAACCAA
CTTCCCTTGCAAATCCTTATTCCTACTAGTTTTCTGATGAGGATTCACTTTCGAAAACCG
ATTATAACTCAACGAAGAAAAAAACGGCACAAAGATAACATCAGCTTCACTAGAATTATA
AACACGCTTCGCCGCAACACTTCTCGGTGCATTCTCATACTCCGATGCAAGCAAATCCAA
CGTAAGCCAATACTCGATACTATGCTGCAAATTCAAACCTCCAGGATAAGGTGGTATATA
CTTCTGAATATCAGGCCATACACTACTATCACTACCTTCCTTCTTCTTCCAATCTAACAA
TCCAAAATGAAACTCTGGATCCATATTATACATATAAACCTTCAAAGGACCCGCGATTAC
CGACGATTTCTCGTCCTTAACACATCTACAATTCGCTTTCTCCGGCCTTACATCGACGAA
TGATTTAGATGGAAATGTAGAGCTAAGGAAGCGATTATGAGCAGTGGAACGTAACACAAA
GTA
   
Sequence Name 74-K112878-0022-973-D03C-24hq
GenBank Accession LN513102 [GenBank]
Sequencing primer gene specific primer, 24hq: 5'-TTACTTTGTGTTACGTTCCACTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K112878-0022-973-D03C-24hq
TTTCATCTAATCATTCGTCGATGTAAGGCCGGAGAAAGCGAATTGTAGATGTGTTAAGGA
CGAGAAATCGTCGGTAATCGCGGGTCCTTTGAAGGTTTATATGTATAATATGGATCCAGA
GTTTCATTTTGGATTGTTAGATTGGAAGAAGAAGGAAGGTAGTGATAGTAGTGTATGGCC
TGATATTCAGAAGTATATACCACCTTATCCTGGAGGTTTGAATTTGCAGCATAGTATCGA
GTATTGGCTTACGTTGGATTTGCTTGCATCGGAGTATGAGAATGCACCGAGAAGTGTTGC
GGCGAAGCGTGTTTATAATTCTAGTGAAGCTGATGTTATCTTTGTGCCGTTTTTTTCTTC
GTTGAGTTATAATCGGTTTTCGAAAGTGAATCCTCATCAGAAAACTAGTAGGAATAAGGA
TTTGCAAGGGAAGTTGGTTACGTTTTTGACTGCTCAAGAGGAGTGGAAGAGGAGTGGTGG
TAGAGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11