Sequences from confirmation amplicon


Confirmation sequences for line 974B06 and hit At1g16530 are shown.
Line ID 974B06
Locus At1g16530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5651589
Primers used for PCR 56xc - 8474, show primer details
   
Sequence Name 77-K118926-0022-974-B06C-56xc
GenBank Accession LN513614 [GenBank]
Sequencing primer gene specific primer, 56xc: 5'-TGTGTATTCCCTACGGGATTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K118926-0022-974-B06C-56xc
TCCAACAAGAAGAAGAGTATCTCGTTTTGGGAAGTGTTGAGTTTCTCTCTTATAATTGGC
TTTGAATAAAGCTTTATATGAAAGTATGTCTAATACACACACTTATCAATATGTAAACAT
ATTCTTTCTCTACAGAATTAAGAAATTATGAGTTCATACAAAAATATATAAAACATTGAA
AGAAGGCAGATTGAAATAAAGTTTACAATCTTAGTTCTTTTAGCTAGTACTTTGCTAATC
TAGACAAATACTTTAATTTAATTGTATATTTATCATGTTCATATTAATTACATACACATC
TTATCATTTATTTATTTTAACTATTGCAAAGATATGATAGAGTGCTAACTAAAGCTAGCA
AGACCAAAGGAAGTCTCCGGCATCATCCGTATACGCAACAGCCATGTCGATGTCACTGTA
GAAGTCTTTGTTCGAGGGGAAAGTAACGGTACTTGCCGTGTACGGCGGGGGTTTGATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CG
   
Sequence Name 53-K118925-0022-974-B06C-8409
GenBank Accession LN508314 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118925-0022-974-B06C-8409
CATTTACATTGAATATAAACCCCCGCCGTACACGGCAAGTACCGTTACTTTCCCCTCGAA
CAAAGACTTCTACAGTGACATCGACATGGCTGTTGCGTATACGGATGATGCCGGAGACTT
CCTTTGGTCTTGCTAGCTTTAGTTAGCACTCTATCATATCTTTGCAATAGTTAAAATAAA
TAAATGATAAGATGTGTATGTAATTAATATGAACATGATAAATATACAATTAAATTAAAG
TATTTGTCTAGATTAGCAAAGTACTAGCTAAAAGAACTAAGATTGTAAACTTTATTTCAA
TCTGCCTTCTTTCAATGTTTTATATATTTTTGTATGAACTCATAATTTCTTAATTCTGTA
GAGAAAGAATATGTTTACATATTGATAAGTGTGTGTATTAGACATACTTTCATATAAAGC
TTTATTCAAAGCCAATTATAAGAGAGAAACTCAACACTTCCCAAAACGAGATACTTCTTC
TTCTTTGTTTGTGATGAAACAAAAAAAACACTTTGCAAACTAAATCCCGTAGGGAATACA
C

Last Updated on 06.12.2018 16:11