Sequences from confirmation amplicon


Confirmation sequences for line SALK_000573 and hit At2g38960 are shown.
Line ID SALK_000573
Locus At2g38960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr:
Primers used for PCR 38us - LBb3, show primer details
   
Sequence Name 94-K117830-SALK_000573C-38us
GenBank Accession LN486358 [GenBank]
Sequencing primer gene specific primer, 38us: 5'-TGGCTTCTTAAATACTTCAGGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K117830-SALK_000573C-38us
CTCTTACCGGCACATGCCATCATCAGGCCAAAACGGGCAATCACACCACAGTTTAACCTA
TTAAACGCATTCAAACCTATCACAACACACCTCCAAATCCTCTAGATACTTTCATGATCA
AAGAAGAAATATGAACCAACCTTGAAATATCTGTAAAATGGTGTCTTAACAAGGTCCTGA
AGCAATGGATTCAACACTTCCGTGTTTAGCCGATTCACAGTTTCATAATCACAACAACAA
TCTTCAACTATCCCAATGTACTTCTGCTGCTCAGCCTAACAACAAAATCAAAACGATTGA
GAAGTCTAGTATACAAATCAAAATCAAAGTAATCGCTGAGATTCAAAGCTAATCACGAGC
TCAAGTATCCAATTCAGAAACTTACAAAGAGCTTTTAGAAATACCTAACCTGACGGCAAT
TGCATATTTTTCCGGTAAATTCAGAAATCGACGAATTCTGAGTATTCAAAAACACAGCGA
CCACGACGGCTAACAGAACGGCAGCTATAGCTCCGATCAGCAAAATCCAGCGCTTTCCCG
ATCCTTTCTCCTTCCCCTTCACACTTCCGACGTCCGTCTCCGCCATTCGCACTATCGCAA
ATAGAACTAAATCTGACACAGACGACTACGACGAGTAATCTCCGGCACTGAATTTTGAAT
TTTCAGAGAAATTGTGGGCCATTTTTACAAAACCGCAGCTCTCANAAAAAGAAAGTCAAA
ACTAATCTCAATCTCATAAAGTCATCANATCCAACGGCTCTGTATTTTCGTCAAATATTT
TCCTTCTACTTTAGTTAGAGTCAACGTAAATAAATATTAGTGAAACCAAAAAAATGTTTT
TGTCTATAAGCCAAAACAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGGTGGTTTTTC
   
Sequence Name 77-K117829-SALK_000573C-R210
GenBank Accession LN486179 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K117829-SALK_000573C-R210
CGTCATTGTTTGTTTGGCTATAGACAAAACATTTTTTTGGTTTCACTAATATTTATTTAC
GTTGACTCTAACTAAAGTAGAAGGAAAATATTTGACGAAAATACAGAGCCGTTGGATTTG
ATGACTTTATGAGATTGAGATTAGTTTTGACTTTCTTTTTTTGAGAGCTGCGGTTTTGTA
AAAATGGCCCACAATTTCTCTGAAAATTCAAAATTCAGTGCCGGAGATTACTCGTCGTAG
TCGTCTGTGTCAGATTTAGTTCTATTTGCGATAGTGCGAATGGCGGAGACGGACGTCGGA
AGTGTGAAGGGGAAGGAGAAAGGATCGGGAAAGCGCTGGATTTTGCTGATCGGAGCTATA
GCTGCCGTTCTGTTAGCCGTCGTGGTCGCTGTGTTTTTGAATACTCAGAATTCGTCGATT
TCTGAATTTACCGGAAAAATATGCAATTGCCGTCGGGTTAGGTATTTCTAAAAGCTCTTT
GTAAGTTTCTGAATTGGATACTTGAGCTCGTGATTAGCTTTGAATCTCAGCGATTACTTT
GATTTTGATTTGTATACCAGACTTCTCAATCGTTTTGATTTTGTTGTTAGGCTGAGCAGC
AGAAGTACATTGGGATAGTTGAAGATTGTTGTTGTGATTATGAAACTGTGAATCGGCTAA
ACTCGGAAGTGTTGAATCCATTGCTTCAGGACCTTGTTAAGACACCATTTTACAGATATT
TCAAGGTTGGTTCAGATTTCTTCTTTGATCATGAAAGTATCTAGAGGATTTGGAGGTGTG
TTGTGATAGGTTTGAATGCGTTTAATAGGTTAAACTGTGGTGTGATTGCCCGTTTTGGCC
TGAAGACGGCATGT

Last Updated on 06.12.2018 16:11