Sequences from confirmation amplicon


Confirmation sequences for line SALK_002445c and hit At1g03700 are shown.
Line ID SALK_002445c
Locus At1g03700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:921679
Primers used for PCR 33wu - LBb3, show primer details
   
Sequence Name 35-K118859-SALK_002445C-33wu
GenBank Accession LN485228 [GenBank]
Sequencing primer gene specific primer, 33wu: 5'-GAGCTGGTCAATCATCATCAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K118859-SALK_002445C-33wu
TTTGTCCTTATAAATTCAACCTGTTCAAAAACACATGCGGAAAAACGAGTTGCTCAAGGT
TAAAACCTCATAAAACGACTAAGGTATATTTCAACACAATTTTTTTTGAAAATGTATTGA
AGTTCATTTCTCATATATATACATAAACAAAAAAAAATACATAATTTTTATTTTATTAAA
CAAATTATTACATAATTTTATATGATCAATCTATCTACTACACTAATAAAAATCCACGTG
TCATAATTTCATGTAATATATTGTCAGTTTAATCTTATCTAGTAACATAACTTTAAATAT
AATCTTATCTATAGAAAAGATAGTATTATTTATTAATTAATGATGATATGATTAACCATA
TGAAATTATGACACGTGACTTAATTTTAAATGAACAACACTAAAATCACAAAAACACATG
TGGTTCCATGAATATTTAGCCTTAAATTTTATATTACTTTGATTCATGATTTTCGAAGAA
GAAAAGGATCAACAACATGGTGGATAGAGAAGATGAGCAAGAAAACGTAGAGAACCAATG
CAACTAAACCGGCAATTAGAGCTCCGGTTATCTGATCGCAAAATCTCGAAACTTGACCGC
ATATTGGCAACCATCCTGCGTTTGCGTTTATATATTGCGGACAAATTGACGCTAAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
   
Sequence Name 11-K118858-SALK_002445C-R210
GenBank Accession LN484599 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118858-SALK_002445C-R210
GTCATTTGTCCGCATATATAAACGCAAACGCAGGATGGTTGCCAATATGCGGTCAAGTTC
CGAGATTTTGCGATCAGATAACCGGAGCTCTAATCGCCGGTTTAGTTGCATTGGTTCTCT
ACGTTTTCTTGCTCATCTTCTCTATCCACCATGTTGTTGATCCTTTTCTTCTTCGAAAAT
CATGAATCAAAGTAATATAAAATTTAAGGCTAAATATTCATGGAACCACATGTGTTTTTG
TGATTTTAGTGTTGTTCATTTAAAATTAAGTCACGTGTCATAATTTCATATGGTTAATCA
TATCATCATTAATTAATAAATAATACTATCTTTTCTATAGATAAGATTATATTTAAAGTT
ATGTTACTAGATAAGATTAAACTGACAATATATTACATGAAATTATGACACGTGGATTTT
TATTAGTGTAGTAGATAGATTGATCATATAAAATTATGTAATAATTTGTTTAATAAAATA
AAAATTATGTATTTTTTTTTGTTTATGTATATATATGAGAAATGAACTTCAATACATTTT
CAAAAAAAATTGTGTTGAAATATACCTTAGTCGTTTTATGAGGTTTTAACCTTGAGCAAC
TCGTTTTTCCGCATGTGTTTTTGAACAGGTTGAATATTATTAAGTGACAAAAATCTTTTT
GTGGGCTCCATTACTTGATGAT

Last Updated on 06.12.2018 16:11