Sequences from confirmation amplicon


Confirmation sequences for line SALK_002536 and hit At5g66940 are shown.
Line ID SALK_002536
Locus At5g66940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26728506
Primers used for PCR 74sm - LBb3, show primer details
   
Sequence Name 34-K116586-SALK_002536C-74sm
GenBank Accession LN485205 [GenBank]
Sequencing primer gene specific primer, 74sm: 5'-TGGAGAGATTAGAGAATCGTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K116586-SALK_002536C-74sm
GAAACAGAGCATATCTATTTCTCTCTCTATGTTCACAATAAACCCAACATACTCTGTCAA
AAAGCTTCACTTTCTTCTCCTTCCCCATCTCTCTCTCTCTCCCTCTCCTCCATAAAAGGA
AACTCTTACTCTATCACAAAGTTCCAACCTTTAACTCTCCAAAGCTTCAAAAACTCTCAA
CTTTCAGATAACCTCTCTTCTCAACAACATGCCTTCTGAATTCAGTGAATCTCGTCGGGT
TCCTAAGATTCCCCACGGCCAAGGAGGATCTGTTGCGATTCCGACGGATCAACAAGAGCA
GCTTTCTTGTCCTCGCTGTGAATCAACCAACACCAAGTTCTGTTACTACAACAACTACAA
CTTCTCACAACCTAATAACACATTGGCCGCTTAATAACACATTGTGGACATTTTAATGTA
CTAACAAATTATTCTAGAATTAAGGCGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTT
   
Sequence Name 17-K116585-SALK_002536C-R210
GenBank Accession LN484756 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116585-SALK_002536C-R210
ATTAGGGTGTGAGAAGTTGTAGTTGTTGTAGTAACAGAACTTGGTGTTGGTTGATTCACA
GCGAGGACAAGAAAGCTGCTCTTGTTGATCCGTCGGAATCGCAACAGATCCTCCTTGGCC
GTGGGGAATCTTAGGAACCCGACGAGATTCACTGAATTCAGAAGGCATGTTGTTGAGAAG
AGAGGTTATCTGAAAGTTGAGAGTTTTTGAAGCTTTGGAGAGTTAAAGGTTGGAACTTTG
TGATAGAGTAAGAGTTTCCTTTTATGGAGGAGAGGGAGAGAGAGAGAGATGGGGAAGGAG
AAGAAAGTGAAGCTTTTTGACAGAGTATGTTGGGTTTATTGTGAACATAGAGAGAGAATA
GATATGCTCTGTTTCTTTACTTCATTATACGTAGCTGGTGGCTCTTCTTTCAAGATTTTC
TGTTTTGAAATAAAACCACGATTCT

Last Updated on 06.12.2018 16:11