Sequences from confirmation amplicon


Confirmation sequences for line SALK_003186 and hit At5g47140 are shown.
Line ID SALK_003186
Locus At5g47140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19145887
Primers used for PCR 16ux - LBb3, show primer details
   
Sequence Name 13-K117668-SALK_003186C-R210
GenBank Accession LN484652 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117668-SALK_003186C-R210
CCTTCTCTTACAGAAAAGTCGAGTCCCACGCATTAGATTGTGCTTAGCTCTCGGAGTTTG
ATACAACCGATCCTGAACTCGATCTATTGCTAGCTTCTTCATCCAGAGCCTTCCTCTTGA
AACCGGTATGGAATTCCAAGTTAGCTCTTTTCACTGTGAAGTTTTCTTGATATGGTTTCC
TCTTGGGAATCTTTTTGTTCAAAGACATTCCCTTAATCATCACCGTTTGAGTTCTATGAT
CCTCAATCTCAGTCTCATCACCTTCAGCACGAGCATGAAGAGGTGTGTAGTTTACTAATG
ATCCTTTAGTTCTCCACCTCGAACCACACGCATTGCACAACACCGGCTTCTCTGGTGGCC
CGTTTCTCCATAGAGGTGTACCTGTTTGCAACATTTTCACAATGATCAAACACTCAAAAT
GAAGAACAATGTTCTTTGAAAACCAAAACAATCATGTGAAGTTTTTAACATCAAAGCAAA
GCAGAGTCTTGATTCAACATAAGTGAGCAATTCTGACACAAAACTATGAGTTCAGTATTC
ATTTGATAACATTCAAAGCTCTCAATAAGAGTGATCAAAA
   
Sequence Name 50-K117669-SALK_003186C-16ux
GenBank Accession LN485516 [GenBank]
Sequencing primer gene specific primer, 16ux: 5'-TGTAGGGTCCTTTTGATCACTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117669-SALK_003186C-16ux
TTTGTGTCGAATTGCTCACTTATGTTGAATCAACACTTTACTTTGCTTTGATGTTAAAAA
CTTCACATGATTGTTTTGGTTTTCAAAGAACATTGTTCTTCATTTTGAGTGTTTGATCAT
TGTGAAAATGTTGCAAACAGGTACACCTCTATGGAGAAACGGGCCACCAGAGAAGCCGGT
GTTGTGCAATGCGTGTGGTTCGAGGTGGAGAACTAAAGGATCATTAGTAAACTACACACC
TCTTCATGCTCGTGCTGAAGGTGATGAGACTGAGATTGAGGATCATAGAACTCAAACGGT
GATGATTAAGGGAATGTCTTTGAACAAAAAGATTCCCAAGAGGAAACCATATCAAGAAAA
CTTCACAGTGAAAAGAGCTAACTTGGAATTCCATACCGGTTTCAAGAGGAAGGCTCTGGA
TGAAGAAGCTAGCAATAGATCGAGTTCAGGATCGGTTGTATCAAACTCCGAGAGCTGTGC
ACAATCTAATGCGTGGGACTCGACTTTTCCTTGTAAGAGAAGGACATGTGTGGGACGTCC
AAAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAC
CCGCCAAA

Last Updated on 06.12.2018 16:11