Sequences from confirmation amplicon


Confirmation sequences for line SALK_004184 and hit At1g14460 are shown.
Line ID SALK_004184
Locus At1g14460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4949658
Primers used for PCR 41kq - LBb3, show primer details
   
Sequence Name 50-K113870-SALK_004184C-41kq
GenBank Accession LN485512 [GenBank]
Sequencing primer gene specific primer, 41kq: 5'-TTGGTTCTTCTTGGAGACTTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K113870-SALK_004184C-41kq
TCCTCTCCTGATTCGATTCATATCCTCTGCACAAACCAAACAAAACAACTGTTTATTATG
AAGATCTTACTTATTCGGTTTTCTCTTCACTGAAGCAAGATTACTTACAGATTGTCTTTG
TTGTTTGTGAAGTTACTATTGTGTAGTAAGCTTGGAGAAACAGGACAATGTTCCATTCTT
TTACCACTCTGATTCTCCTGAAACTCACCCATTTCACAAATCTTCAAGACTTTAACTCCA
TTGGTGTCTTCTTGAGGTAGAATTTGGTTCCTTTCGGGTTTTAACCGGCCTTGTGAACCA
GGAGTCTTCTGTAACCACTCAGTTTCTAATCTCTGTTCTTGGATGATTGCTTCAATCCTT
TTCATTGGAATCTCATTCCCACTTTCAGTATATGAACTAGTTGTCACTGCTATTTGTCTG
GTCTGCTTGGAGTTGAGTAACTCAGTTTCAGACAAGAGGATTATCAGGATATATTGTGGT
GTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTCCGA
   
Sequence Name 58-K113869-SALK_004184C-R210
GenBank Accession LN485691 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113869-SALK_004184C-R210
CATTTGTTTCACCACAATATATCCTGATAATCCTCTTGTCTGAAACTGAGTTACTCTACT
CCAAGCAGACCAGACAAATAGCAGTGACAACTAGTTCATATACTGAAAGTGGGAATGAGA
TTCCAATGAAAAGGATTGAAGCAATCATCCAAGAACAGAGATTAGAAACTGAGTGGTTAC
AGAAGACTCCTGGTTCACAAGGCCGGTTAAAACCCGAAAGGAACCAAATTCTACCTCAAG
AAGACACCAATGGAGTTAAAGTCTTGAAGATTTGTGAAATGGGTGAGTTTCAGGAGAATC
AGAGTGGTAAAAGAATGGAACATTGTCCTGTTTCTCCAAGCTTACTACACAATAGTAACT
TCACAAACAACAAAGACAATCTGTAAGTAATCTTGCTTCAGTGAAGAGAAAACCGAATAA
GTAAGATCTTCATAATAAACAATTGTTTTGTTTGGTTTGTGCAGAGGATATGAATCAGAA
TCAGGGAGAGGAGTTTGCAGTCTGCTTTTCTGTTGGAACACACAAAAGTCTCCAGAAGGA
ACC

Last Updated on 06.12.2018 16:11