Sequences from confirmation amplicon


Confirmation sequences for line SALK_004777c and hit At3g03660 are shown.
Line ID SALK_004777c
Locus At3g03660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:891489
Primers used for PCR 75sm - LBb3, show primer details
   
Sequence Name 36-K116586-SALK_004777C-75sm
GenBank Accession LN485238 [GenBank]
Sequencing primer gene specific primer, 75sm: 5'-CCGTTATAGCCCCTGTATTACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116586-SALK_004777C-75sm
GCATTATTCGAAGAAGAACTAAGCTACTACTAAAAACGGTCAGTTTCTTTGGTTAAACTC
ATCTGAATTTTTTTTAATTTCTTATCGTTGTCTTTAAAAGAACTTTTACAAGTAAAATTC
GAGATCTAGAGATGTTTTTTTCCTGGAAAAAAAAACCTTCATAGAGAACTTGCTTTCAAG
TACAAAATGTTAGGCAATATTGGAAGCATGATTAACCAAGAAGCATTAAACAAAAAAAAA
ATTAAAAAAAATTACCCAAAAACCATTTTTTACCTATTTCCCCAATTAAAACCAATTCGT
TTTTCCGGTTGAACCCCCCCCAAAATTGTTTTTTTTCCTCCTCTAAACCACCTTTTCCAT
TTAAAAAATATTTATTTTTAAAAACTTTTTGGGAAAAAAAAAAGGTTTTAAAGGATAACC
CCTTTTCCTTTCCAAAAAAAAAAAAATTCTTGCAAAAAATGTTTTTCCTTTTGTTACCCT
GGTGGGTATTGAAATTTGAATTTTTAATCAAATGGCCCCAAATTCATTTTGACTTTTGAC
ACCTTAATACCCCTTTGCGAACTTTTTTAATGTCCGGGGGTGTTTTTTCTTTTCACCATT
GAAACGGGCAACACTTAATTCCCTTTCCCCCCCGGCCCTTAAAAAATTTCCACCAACCGT
TCCACCCTGTTTTCCCCCACCGGGCAAAAATCCTTTTTAATG
   
Sequence Name 19-K116585-SALK_004777C-R210
GenBank Accession LN484827 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116585-SALK_004777C-R210
AAAACTCCAATTCTTAATACCAACAAGGGTAAAACAAAAGAAAAAACATATTTTGCACGA
CTCTTTTTTTCTTTGTAAGCGAAAGTCGGTTTATCCTCTAAACCCTCTTTCTTTCCCCAA
AAAGTCTCTAAAACTAACTATTTTTTAATTGTAAAGGTTGTTTTAGAGTAGGAAAAAAAT
CAATTCTGGGTGGGTTTCAACCGGAAAACCGATTTGTTTTTATTTGGCGACTTAGTTAAT
AATTGCTTCTTGGTTATTTTTTTTTATTTTTTTTTTTGTTAAAGGCTTCTGGGTTAACCA
GGCTTCCAATATTGCCTAACTTTTTGTATTTGAAACCAATTTCTCTTTAAAGGTTTTTTT
TTCCGGGAAAAAAACTTCTCTAAATCTCATTTTTCCTTGAAAAATTTTTTTTAAGGCCAC
CTTAAAATTTAAAAAATTTCAAATGATTTTACCCAAAAAATTGCCCTTTTTTAGTAGTAC
CTTATTTCTTCTTCTAAAAAGGCCAAATCTTGTTTTTGAAAACTTTTTTTTTTTAACCTT
TTTTTCTTCCATTTTATTAAAAATTTATTTTTT

Last Updated on 06.12.2018 16:11