Sequences from confirmation amplicon


Confirmation sequences for line SALK_005080c and hit At3g22820 are shown.
Line ID SALK_005080c
Locus At3g22820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8074117
Primers used for PCR 76sm - LBb3, show primer details
   
Sequence Name 38-K116586-SALK_005080C-76sm
GenBank Accession LN485269 [GenBank]
Sequencing primer gene specific primer, 76sm: 5'-ATTGCCTCAAAGCTAACCAGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K116586-SALK_005080C-76sm
CGAGGAAAGAAAAGCTAACAAAAGTTCACTAATTGTTTGAAGAATTAATTAAGTTAGGTC
ATGAAGATCTGTTTTTGGTTTTAGGGCATGAAGAGTTTGTTGCCACACTTGCAGCGCCAA
GCTTCAGGGTAATACTCCAGTGGCATAATCAGACCTGGTTGAATCGGAACGTGAACTGCT
TTACAAGGCTCACATTTCCCACACTTCAATCTACACATCGGAGGCACCGAGCCAGGTCCC
CCTAACCGCTTCTGATCTACAATCTGACCCGGTAAACCCGACCTAGCTATCTCCTTCTTC
CCCTGCCCAAGCCCTCCACCTGGTTTAACGACAGAGTAAATTCATGACTACTAGTAAATA
GGCAAAAGAAACAAAAAATGGGGTTTTCACTTTTTCTCCTTCATTTCTTCTTTTGTTTAA
GCAACAAAACATTTTGCTTAGTTGAGTATGCAATTGTTAGAAGTTAAGATCTTTTAGGTT
CCTATTCCACACATAACTATACAGAAAAAACGGTTCCATATAGATTTTTTTTTCAAAACA
AACAAACTATGCTATGTAGATAAAACATGATAGTAGATAAAAGAGTTTAAAGAAGTGGGT
TCTTACTGGGACGTTGAAGAGAGGCGGCGGAGGACGACGAGAAGAAGAGAAGGAAAGCAT
AAACGATGAGAGTTGGGAGGACGACGCCCATTAGCCAGGACCGGTGAGGAGTGTTTTTGT
CTTTAGGTTTAGGAATTTTGGGTAAAGTGGTCCAAAGTCAAAGAGTAATCAAATTGACGC
TTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCANGCGAAAATCCTGTTTGATGGTGTCACCCCC
CCCAAAAA
   
Sequence Name 21-K116585-SALK_005080C-R210
GenBank Accession LN484891 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116585-SALK_005080C-R210
CTTTACCCAAAATTTCTAAACCTAAAGACAAAAACACTCCTCACCGGTCCTGGCTAATGG
GCGTCGTCCTCCCAACTCTCATCGTTTATGCTTTCCTTCTCTTCTTCTCGTCGTCCTCCG
CCGCCTCTCTTCAACGTCCCAGTAAGAACCCACTTCTTTAAACTCTTTTATCTACTATCA
TGTTTTATCTACATAGCATAGTTTGTTTGTTTTGAAAAAAAAATCTATATGGAACCGTTT
TTTCTGTATAGTTATGTGTGGAATAGGAACCTAAAAGATCTTAACTTCTAACAATTGCAT
ACTCAACTAAGCAAAATGTTTTGTTGCTTAAACAAAAGAAGAAATGAAGGAGAAAAAGTG
AAAACCCCATTTTTTGTTTCTTTTGCCTATTTACTAGTAGTCATGAATTTACTCTGTCGT
TAAACCAGGTGGAGGGCTTGGGCAGGGGAAGAAGGAGATAGCTAGGTCGGGTTTACCGGG
TCAGATTGTAGATCAGAAGCGGTTAGGGGGACCTGGCTCGGTGCCTCCGATGTGTAGATT
GAAGTGTGGGAAATGTGAGCCTTGTAAAGCAGTTCACGTTCCGATTCAACCAGGTCTGAT
TATGCCACTGGAGTATTACCCTGAAGCTTGGCGCTGCAAGTGTGGCAACAAACTCTTCAT
GCCCTAAAACCAAAAACAGATCTTCATGACCTAACTTAATTAATTCTTCAACAATTAGTG
AACTTTTTGTTAGCTTTTTCTTTTCCTCGGGATTAATTTTGTCCTCCTTACTAATTGGTT
TTTACTGG

Last Updated on 06.12.2018 16:11