Sequences from confirmation amplicon


Confirmation sequences for line SALK_005722c and hit At2g41010 are shown.
Line ID SALK_005722c
Locus At2g41010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17114591
Primers used for PCR 43et - LBb3, show primer details
   
Sequence Name 23-K111273-SALK_005722C-43et
GenBank Accession LN484945 [GenBank]
Sequencing primer gene specific primer, 43et: 5'-TTTCTTTTTCTTTTTGGGGCTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K111273-SALK_005722C-43et
AGTTGAGATATTCTTATTGGATCATCAATATTGCAAAGGAGATTGTCTGAATATTGGATA
TACGCAATAAAAAAAAATTTCTTTCGCTTGTTACAGAGTACATACATACTATCCACATGT
TATCCATTCGATTTTGTATAAGTCTGACATAATTCATCTTTAACAAAAAAAAAAAAATTC
GGGAACAAATTCAAAAAACCAATTTGGTTTAAAAAAAACCCCCCCCCCTTAACCAAAAAA
AAATTCCCAAAAAAAAAACTGGGAACCGGGGCCCTTTTAAATCCCCGTTAAAGGGGTTTC
CAATCGTTTCCCCCCGCCTTTATAAAAATTCCCCGGCCCCCTTTTTTATTTTCCCCCCTC
TCTTTTTTTTTCCAAAATTTCTCTTTCTCCCCTTTAAAAAACCCCGGGAACATTTTTTTT
CCCGGGTAAGGGGAACTTCGAAGGAATAACAAGGGTTTATTTTTGGTTTATTTCCCCAGG
GTTCAACCGTAAATCCGGGGTGGTTTTTAAAACCTTTCTCTCAAAAAAAAATTTTGTTCA
AAAAATTTTTCAAAACCCCGGTAAAACCCCCTAAAACTTTAATTCTTAAAAAACAATTGG
GGAACTTTTTAAAGGAAGGGGGGGGTTTTTTTTTTCCCCCGGGAAAAGGGGAAACATTTA
ATGCCCTTTCCCCCCGGGCCCTTAAAAAATTTCCACAAGGGGTCCCACCTGGTTTGCCCC
CACCGGGAAAAAACCCGTTTGGAGGGGGTTCC
   
Sequence Name 07-K111272-SALK_005722C-R210
GenBank Accession LN484456 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K111272-SALK_005722C-R210
GGCTGTGACGGTGGTATGGAGAGCTCTTGCGAGCAAATCGTTGTCGTGAGAGAAGGTATC
AGAAAGCAACCATGAATCAACGTTGAAACCTTGGCGATATAACCAAGAATCAACGCTTGC
TAATCCCTCCGAAGTCACCATAACCGGTGAAGAAGATGGTCGCTGGGTTTCTGAAAGGAG
AAGAAGAAGAAGTTAGAGAAAGAAAGAGAGAGGTGAGAAATGAAAAATGGGGCCGAGGAA
GTTTATATAAGGACGGCGAGAAGCGATGGAAACCGCGTTGAGCGGAGATTGAAAGGGACA
CGTGTCACAGCTTACTCGTACGGAACTTTTATTCAGCTAACGAGTGAGAGATTATCATAA
AACAAATTAGATTATTGAATTATGTCACAGATTTTTTTTTTTTTTTTAAAAAAGAATTTT
TGTCAAATTTTTCCAAATCCAAGGGGAAAACGGGGGAAAAGAAGGTTTGTCCTCGGAAAC
AGGCAAAAAAATTTTTTTTTTTTGGGGTATTCCCAATTTTAAAAAATTCCCTTTGGAAAT
TTGGTTGGCCCCAAAAAAAATTTTTCCACTTTTAAAAACCTCCTTTTCACCCGGGGGAAA
CTGGAAATTTCCCCCCAAAAAAAAAAAAAAAAGGGGGNTTTTTTTT

Last Updated on 06.12.2018 16:11