Sequences from confirmation amplicon


Confirmation sequences for line SALK_006926c and hit At2g45880 are shown.
Line ID SALK_006926c
Locus At2g45880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18882557
Primers used for PCR 06oz - LBb3, show primer details
   
Sequence Name 18-K115143-SALK_006926C-06oz
GenBank Accession LN484787 [GenBank]
Sequencing primer gene specific primer, 06oz: 5'-TTTGTGACTAACGAAACCAAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115143-SALK_006926C-06oz
ATATCATTGACCTCTGTGAGTAAGAGACACTAAGCACAATTCTTTCAACAATCACAAGCC
TCCTCTCGAAATTGGTCAGAATTCTCGGCCCTCTGGGCGAATCATCGAGCAATACTAAAA
AACCCTATAAATGGAGCTTCCGATCAAAGGTTTCCCCCTCTGTCGCCGTCAGCGAAACCA
AGAGAGAGGAGAAGAAGACGGTGGATCTCCGGCGGGTGAAAAGAGGCGGATCTGGCCGTG
AGATGGAGGAAGCGCGTGAAGGAGCGTGCAGCGTGAATTGGAAGACGAGCTTTCACGCGT
GTGTAGCGCTCGAGCGTGTCTGAGAGAAAGAGGAGCCCGTCTCCCAGGATATATTGTGGT
GTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTCCGAAAT
   
Sequence Name 02-K115142-SALK_006926C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115142-SALK_006926C-R210
AAATAGTCGCGGATTGGTTTCATGAACCGCGGAAGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAA

Last Updated on 06.12.2018 16:11