Sequences from confirmation amplicon


Confirmation sequences for line SALK_012200c and hit At1g78450 are shown.
Line ID SALK_012200c
Locus At1g78450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29517191
Primers used for PCR SC08 - R204, show primer details
   
Sequence Name 49-K106282-SALK_012200C-R210
GenBank Accession LN485469 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K106282-SALK_012200C-R210
GCGTCATTTGTTACCCACAATTTGAAATGGAAGATGAAGAAACTGTTAGTATCGTTACTC
AGTGAACAAAAAAATGAACACTTTTTTATCTTTTTATGGTGTAGTGACAAAGCAAAGCCA
TTGAGATAAGCCTATCTCCTAAAAGTTGTTAAATAACTGTCCATTGTCTTACTCTGGTCA
GCACATTCCCTAAAGAAAAGAAAATCAAAAGAGTACTTTTTTCTGATAAGTGATAACCCA
CCTGAGGCTACATACTCAATGCAACTCACAGCACCTATGTAAGAAATGAGAGCTATCTAT
ATAATCGAACAAAACTTCTAATCAATGAACTCCTGATTACGCGTTTGTTCCAGACCAATA
GAAAACTCAAATCCACAAAGAACCTAGCATATTATAAGACCGCTTAATAGAAACACCCCT
CAAGAAAAGACCCGAATTCTTGAATCACCTGGTTTATTTACAACGATGAAGACAACAAAA
GAGCAGAAGAAACCCTAATTTTCTCAATCTTTTGATGTTAACATGAGCAGGAGAGTAAAA
GAAAGATTTACTGATTTCAAGAGAGACAAAATCCCCACAGACTTTTCAAATGTTCTAAGA
ATGACATAATGCAAAAAGTTTTGAAAATGACACTCCAATAACACATATACTGTATACATA
CACTCTAATAGTGATCGTATTTGCTTTTGAAAACGCTACTCAATCAGATTTAAGAAAAAA
TAAGACTTGAAATCTGTGTTATGTCAAGCATAATACTGAAAACTCTGTCTATATNTCATT
TTTATTCTATAGATAGTCCCTTTTTTTGCCAGCATTTATACGGGTCATAATAGGTTAACA
TGACAGTTTTATACTAGTACTATNTTTCATTATTGAACTTTTATTTGGAAGAAGGAAAAT
TAGGAGTCAGACAGAGAATCATATAGGGACGTGGCATATAGCCACTCCCCACGTGGCTCG
AAGCAACTTGATACAAAACATGTCAAATNGGGGTCATTTTT
   
Sequence Name 65-K106283-SALK_012200C-SC08
GenBank Accession LN485863 [GenBank]
Sequencing primer gene specific primer, SC08: 5'-ATCTTCGAGAAGAATGGTACGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K106283-SALK_012200C-SC08
GTTTTGATCAGTGGCTTCGAGCCCGTGGGGAGTGGCTATATGCCACGTCCCTATATGATT
CTCTGTCTGACTCCTAATTTTCCCTCTTCCAAATAAAAGTTCAATAATGAAAAATAGTAC
TAGTATAAAACTGTCATGTTAACCTATTATGACCCGTATAAATGCTGGCAAAAAAAGGGA
CTATCTATAGAATAAAAATGAAATATAGACAGAGTTTTCAGTATTATGCTTGACATAACA
CAGATTTCAAGTCTTATTTTTTCTTAAATCTGATTGAGTAGCGTTTTCAAAAGCAAATAC
GATCACTATTAGAGTGTATGTATACAGTATATGTGTTATTGGAGTGTCATTTTCAAAACT
TTTTGCATTATGTCATTCTTAGAACATTTGAAAAGTCTGTGGGGATTTTGTCTCTCTTGA
AATCAGTAAATCTTTCTTTTACTCTCCTGCTCATGTTAACATCAAAAGATTGAGAAAATT
AGGGTTTCTTCTGCTCTTTTGTTGTCTTCATCGTTGTAAATAAACCAGGTGATTCAAGAA
TTCGGGTCTTTTCTTGAGGGGTGTTTCTATTAAGCGGTCTTATAATATGCTAGGTTCTTT
GTGGATTTGAGTTTTCTATTGGTCTGGAACAAACGCGTAATCAGGAGTTCATTGATTAGA
AGTTTTGTTCGATTATATAGATAGCTCTCATTTCTTACATAGGTGCTGTGAGTTGCATTG
AGTATGTAGCCTCAGGTGGGTTATCACTTATCAGAAAAAAGTACTCTTTTGATTTTCTTT
TCTTTAGGGAATGTGCTGACCAGAGTAAGACAATGGACAGTTATTTAACAACTTTTAGGA
GATAGGCTTATCTCAATGGCTTTGCTTTGTCACTACACCATAAAAAGATAAAAAAGTGTT
CATTT

Last Updated on 06.12.2018 16:11