Sequences from confirmation amplicon


Confirmation sequences for line SALK_015230 and hit At5g60550 are shown.
Line ID SALK_015230
Locus At5g60550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24341661
Primers used for PCR 26du - LBb3, show primer details
   
Sequence Name 58-K110715-SALK_015230C-26du
GenBank Accession LN485686 [GenBank]
Sequencing primer gene specific primer, 26du: 5'-GATTATGAACCATTGGAGGCTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K110715-SALK_015230C-26du
TAGATCCAGGAAGCTTGATCATATGTAAACCAACCTTGTCGTAGGTGTCCTGAAGAGTAT
CCGCAAGAAACGGGTATTGTCCCAATATCATACAATACAATGTAACTCCCACTGCCCATG
TATCCGCAGCTCTGCCACTATACGTTATACCTGATACTGATTTTCATCCAAGAAAAATGA
TACTCGTTGATTCTCCATTCACTTAGACTCAGGACCAAGCTTAGAGAAATTAGAAAGGAA
ACTGACCTAAACAACATTCTGGAGCAGTGAAGACAGGAGTCCCGGGAGATCGGCCGATAT
ATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGGTGGTTCCAAATCGNGCAAAA
   
Sequence Name 42-K110714-SALK_015230C-R210
GenBank Accession LN485330 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K110714-SALK_015230C-R210
CGTCATTTGTTTCACCACAATATATCGGCCGATCTCCCGGGACTCCTGTCTTCACTGCTC
CAGAATGTTGTTTAGGTCAGTTTCCTTTCTAATTTCTCTAAGCTTGGTCCTGAGTCTAAG
TGAATGGAGAATCAACGAGTATCATTTTTCTTGGATGAAAATCAGTATCAGGTATAACGT
ATAGTGGCAGAGCTGCGGATACATGGGCAGTGGGAGTTACATTGTATTGTATGATATTGG
GACAATACCCGTTTCTTGCGGATACTCTTCAGGACACCTACGACAAGGTTGGTTTTACAT
ATGATCAATGCTTTCCTTGGATCTTAAGAACTCTTTTTGAAAATCTTTTAGCCTCCAATG
GTTCATAAT

Last Updated on 06.12.2018 16:11