Sequences from confirmation amplicon


Confirmation sequences for line SALK_015570 and hit At4g22750 are shown.
Line ID SALK_015570
Locus At4g22750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11949989
Primers used for PCR 15im - LBb3, show primer details
   
Sequence Name 14-K113191-SALK_015570C-15im
GenBank Accession LN484671 [GenBank]
Sequencing primer gene specific primer, 15im: 5'-GCTGTTGGTTTCTTGTGTGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K113191-SALK_015570C-15im
ACTCCAGAGGAAGAGTAAGGAAGCAAAGGACTCATTTTTTTTATGATTGTTGTTTTGTTT
GCAGCTTATAATGCTACTGTGGAGTTATTTCTCTGTTGTTGTGACGGACCCTGGTGGTGT
TCCAACGGGTTGGAGGCCAGAATTAGACATAGAGAAAAGCGAGGGAAACCAAGCTTTGAT
TGGAGAAGCTTCGGTTGGAGATTCCTCGTCTCATGGTGTAAGATATTGTAGAAAGTGCAA
TCAATATAAACCGCCTCGTTCTCACCATTGTTCAGTCTGTAAGTCTCTTCAATGAGTGAT
TCTTTTCTTTGAATCCGGTATATGTGTTTTTTAGTAGTGAAAAAGTTGTCATATTGGGTT
TTCAGGTGGAAGATGCATACTAAAGATGGACCATCACTGTGTTTGGGTGGTGAATTGTGT
TGGTGCAAATAACTACAAATCATTTCTCCTATTCTTGGTATGTCAACTTATCATAGCATC
GCTGAACGAAGTTGTTTTTGATATGTTCTGTTCATTAATGTTGCCATGATCATAATCCAT
TCCCAATATCTCAAAAATGCAGTTTTACACATTTCTTGAGACAACGGTGGTTGCAGTATC
ACTATTGCCAATTTTCCTCGTATTCTTCAGCGATGGAGATGGTGATATAACCGTATCACC
AGGAAGCCTAGCAGCCAGCTTTGTTGCATTTGGGTTCAAAACAAATTGACGCTNAGACAA
CTTAATAACACATTGCGGACGTTTTTTATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
   
Sequence Name 03-K113190-SALK_015570C-R210
GenBank Accession LN484340 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K113190-SALK_015570C-R210
TGCAACAAGCTGGCTGCTAGGCTTCCTGGTGATACGGTTATATCACCATCTCCATCGCTG
AAGAATACGAGGAAAATTGGCAATAGTGATACTGCAACCACCGTTGTCTCAAGAAATGTG
TAAAACTGCATTTTTGAGATATTGGGAATGGATTATGATCATGGCAACATTAATGAACAG
AACATATCAAAAACAACTTCGTTCAGCGATGCTATGATAAGTTGACATACCAAGAATAGG
AGAAATGATTTGTAGTTATTTGCACCAACACAATTCACCACCCAAACACAGTGATGGTCC
ATCTTTAGTATGCATCTTCCACCTGAAAACCCAATATGACAACTTTTTCACTACTAAAAA
ACACATATACCGGATTCAAAGAAAAGAATCACTCATTGAAGAGACTTACAGACTGAACAA
TGGTGAGAACGAGGCGGTTTATATTGATTGCACTTTCTACAATATCTTACACCATGAGAC
GAGGAATCTCCAACCGAAGCTTCTCCAATCAAAGCTTGGTTTCCCTCGCTTTTCTCTATG
TCTAATTCTGGCCTCCAACCCGTTGGAACACCACCAGGGTCCGTCACAACAACAGAGAAA
TAACTCCACAGTAGCATTATAAGCTGCAAACAAAACACCAATCATAAAAAAAATGAGTCC
TTTGCTTCCTTACTCTTTCCTCTGTGAGTTCTACAATGCCGAGGGATTTCAATGTTGATA
ATCTATTAAAACCCCC

Last Updated on 06.12.2018 16:11