Sequences from confirmation amplicon


Confirmation sequences for line SALK_016021c and hit At3g42950 are shown.
Line ID SALK_016021c
Locus At3g42950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15015795
Primers used for PCR 16gg - LBb3, show primer details
   
Sequence Name 19-K112129-SALK_016021C-16gg
GenBank Accession LN484815 [GenBank]
Sequencing primer gene specific primer, 16gg: 5'-CTCTCACTCAATCGCTTTCTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112129-SALK_016021C-16gg
CAACAATCAAACACCAAAGTTTGTGTCTTCCCTTAAGATTCACTCAAATCTCGCAACACA
CACATTCAACAAATCCTGATTCATAACATCTCACCAAATCTCTCAAAAGATCTCCAAATC
TAAAATGTTCTTCTTCTTCTTCTTCATCTGAATCCAAAACAACAATGGTGAGACGTTTCC
TCTCTTCTCACAAATCTCTCATCACTGTTTTCTGGATCGCCACTTTCGCTTCCTTATTCA
TCTGGCAATTCGGCGGCGTTTCCACAAATCTCTACTCCGGTTTCTCCCTTTTCTGGTCGT
CGTCTTCAACAACCACCACCGTCTTCTCCGGTGGATTCCCCAAGCTCAGACCTGTAGTTT
TCAATCTAACAGACTTCGGTGCTGTCGGCGATGGTGTCACAATCAACACTGAAGCTTTCG
AGAAAGCTATTTATAAGATCTCTAAGCTTGCTAAGAAAGGAGGTGGTCAACTTAATGTTC
CTCCTGGTCGTTGGCTCACTGCTCCGTTTAATCTCACTAGTTATATGACTCTNTTCCTCT
CTGAGAACGCTGAGATTCTTGCTCTTCAGGTTTTGTTTCCAGGATATATTGTGGTGTAAA
CAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTC
   
Sequence Name 03-K112128-SALK_016021C-R210
GenBank Accession LN484337 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112128-SALK_016021C-R210
TTTGTTTACCCACAATATATCCTGGGAACAAAACCTGAAGAGCAAGAATCTCAGCGTTCT
CAGAGAGGAAAAGAGTCATATAACTAGTGAGATTAAACGGAGCAGTGAGCCAACGACCAG
GAGGAACATTAAGTTGACCACCTCCTTTCTTAGCAAGCTTAGAGATCTTATAAATAGCTT
TCTCGAAAGCTTCAGTGTTGATTGTGACACCATCGCCGACAGCACCGAAGTCTGTTAGAT
TGAAAACTACAGGTCTGAGCTTGGGGAATCCACCGGAGAAGACGGTGGTGGTTGTTGAAG
ACGACGACCAGAAAAGGGAGAAACCGGAGTAGAGATTTGTGGAAACGCCGCCAAATTGCC
AGATGAATAAGGAAGCGAAAGTGGCGATCCAGAAAACAGTGATGAGAGATTTGTGAGAAG
AGAGGAAACGTCTCACCATTGTTGTTTTGGATTCAGATGAAGAAGAAGAAGAAGAACATT
TTAGATTTGGAGATCTTTTGAGAGATTTGGTGAGATGTTATGAATCAGGATTTGTTGAAT
GTGTGTGTTGCGAGATTTGAGTGAATCTTAAGGGAAGACACAAACTTTGTGTGTTTTGAT
TTGTTTGTTGAAACTAAACAAACGTCTCANAGAGAAAGCGAT

Last Updated on 06.12.2018 16:11