Sequences from confirmation amplicon


Confirmation sequences for line SALK_018512c and hit At3g02320 are shown.
Line ID SALK_018512c
Locus At3g02320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:472985
Primers used for PCR 27du - LBb3, show primer details
   
Sequence Name 06-K114130-SALK_018512C-R210
GenBank Accession LN484435 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114130-SALK_018512C-R210
CTTACCGGTCTTCTTTTTGATGGTTTTTTCTTGGGTGATATTGTAGGTGAAGAATCATCC
CATAAAGGCGCAATCGCCTGAACAACCCGGTAGTGTGATCTTGTCCAAAGAACCATCACA
TGAGGTCTTTTGCTCGCTTTCTCTGAATTGTTTGAAACTGAATAATGTTTTTTCCAAGCC
TGAATCTTTTCTTTTCGATTCTGTCTTGTGTTTGGACAGGTTGATTTTTCGCGTCACATT
GGTTCGCTGAGCAAGGCACAGGCAAAGAAAGTAGCTCGTTTTCTGCCAAATCCAGAGAAG
CATTGGGGTCCAAAGCTTAGGGCTGGTCGCCAGATTACGAGCAAACATGTATCTCTTATT
GGTCATGAAGCAGTCAATGGTCATCTTAGCCAACACCATGAAGAACTTAAAGAAGAAGAT
GAAGAAGCAGAGCCNGAAGATAATGTCCAAGA
   
Sequence Name 16-K114131-SALK_018512C-27du
GenBank Accession LN484723 [GenBank]
Sequencing primer gene specific primer, 27du: 5'-GTCTTGGACATTATCTTCTGGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114131-SALK_018512C-27du
TTGGCTAAGATGACCATTGACTGCTTCATGACCAATAAGAGATACATGTTTGCTCGTAAT
CTGGCGACCAGCCCTAAGCTTTGGACCCCAATGCTTCTCTGGATTTGGCAGAAAACGAGC
TACTTTCTTTGCCTGTGCCTTGCTCAGCGAACCAATGTGACGCGAAAAATCAACCTGTCC
AAACACAAGACAGAATCGAAAAGAAAAGATTCAGGCTTGGAAAAAACATTATTCAGTTTC
AAACAATTCAGAGAAAGCGAGCAAAAGACCTCATGTGATGGTTCTTTGGACAAGATCACA
CTACCGGGTTGTTCAGGCGATTGCGCCTTTATGGGATGATTCTTCACCTACAATATCACC
CAAGAAAAAACCATCAAAAAGAAGACCTGGTAAGATGAAACACGGTCACATGACGCTNAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGATTCCGA

Last Updated on 06.12.2018 16:11