Sequences from confirmation amplicon


Confirmation sequences for line SALK_020762 and hit At2g42370 are shown.
Line ID SALK_020762
Locus At2g42370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17644300
Primers used for PCR 35wm - LBb3, show primer details
   
Sequence Name 34-K118709-SALK_020762C-35wm
GenBank Accession LN485210 [GenBank]
Sequencing primer gene specific primer, 35wm: 5'-AAGCTTGTACCTTTCAAGCCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118709-SALK_020762C-35wm
CTCTTAAGACTCTCGGATTGTGGGATTTTGTGCATTTGGAATTTGATCAGGATATGGATT
ATGATCTTGTGGCTCAACTTATAGCTTCCTATAGTGCTGGAGGTAAGTGCAGCTATATAA
ACGGGTCTAGGATTAAACTTAGTCGTGCGGATTTGGCTCGATCTTTGAAGTTGCCTAATA
AGAAAGAACGTGTTGTCATTCTTGATGAGGATAAGGAATTTCTGGAATCAGATGAATCAA
TCAGTGTTGTTGAAGATGTTATCTCTAATTGGATGCTACTGCATTGTGATGATGCGTGGA
TGATGCCAGATGAAGTGGTGGAATGGATGAAAGGTATAAAGAAAAAGCAATTGGATAAGC
TGGACTGGGCTGGACTGATGTGGTTTATGGTTGAGAAAGAGTTGAAAGCGGAGCCTCCAC
TCGGGGATTGTTTCTATGCGTCGCATTTGCAGATGGTCATTAGATCACAGAAAATAGATC
TGTTTAAGGAGAGGGATTTGAAGGTAAAGGATGATATTGCTGCTTTGAATCTAGGAATGG
ATGATGGAGCCACTGACTCAAAGAAAGAAAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGATTGATGGTGGTTCCG
   
Sequence Name 09-K118708-SALK_020762C-R210
GenBank Accession LN484536 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118708-SALK_020762C-R210
CTCCATCATCCATTCCTAGATTCAAGCAGCAATATCATCCTTTACCTTCAAATCCCTCTC
CTTAAACAGATCTATTTTCTGTGATCTAATGACCATCTGCAAATGCGACGCATAGAAACA
ATCCCCGAGTGGAGGCTCCGCTTTCAACTCTTTCTCAACCATAAACCACATCAGTCCAGC
CCAGTCCAGCTTATCCAATTGCTTTTTCTTTATACCTTTCATCCATTCCACCACTTCATC
TGGCATCATCCACGCATCATCACAATGCAGTAGCATCCAATTAGAGATAACATCTTCAAC
AACACTGATTGATTCATCTGATTCCAGAAATTCCTTATCCTCATCAAGAATGACAACACG
TTCTTTCTTATTAGGCAACTTCAAAGATCGAGCCAAATCCGCACGACTAAGTTTAATCCT
AGACCCGTTTATATAGCTGCACTTACCTCCAGCACTATAGGAAGCTATAAGTTGAGCCAC
AAGATCATAATCCATATCCTGATCAAATTCCAAATGCACAAAATCCCACAATCCGAGAGT
CTTTAAGAGACTCTCATAACAAGCAAAATCTAGGGTTTTTAATGGCTTGAAAGTAACAAG
CTT

Last Updated on 06.12.2018 16:11