Sequences from confirmation amplicon


Confirmation sequences for line SALK_022835c and hit At3g10860 are shown.
Line ID SALK_022835c
Locus At3g10860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3400247
Primers used for PCR 11rb - LBb3, show primer details
   
Sequence Name 68-K116055-SALK_022835C-11rb
GenBank Accession LN485961 [GenBank]
Sequencing primer gene specific primer, 11rb: 5'-CTGATGATGAACCGAGTCAGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K116055-SALK_022835C-11rb
CAAATGGCTCACAGATAGAGTAGTTGTCTTGCAGACAAGGAACGTGGAAGATCTTCATCG
TCTCCTTAGATCGATCGTCCTTCTCCTCCGGAACACTAGCTGTATCATCATCATCATTTA
TGTCTGGACTAGTGATTTATCTATTTCGGTTTACTCAAACCGGATCGAACCGAAACAAAT
CAGGATAAATTTTGCAGCCGGTCATCCGGTTCAATTGTAATTGTAACTGAACCGGAATCG
GATAAAAAACATAAGCTTCGTTGAGAATTGTTTATTTTAGGTTTTGGGGGAGATTCGTTC
TCGTACTGTGGATTGTTGTTGTTGGGATTATCTGGGAAGAGAAAAAGAAAATGGGGAAGC
AGCCGGTGAAATTGAAGGCGGTGGTTTACGCACTTTCTCCGTTTCAGCAGAAGATCATGA
CCGGTCTCTGGAAGGATCTGCCGGAGAAGATCCACCACAAGGTCTCTGAGAATTGGATCA
GCGCCACTCTTCTCGTTACCCCTGTCGTTGGAACCTACTGGTACTCTATCTCTCTTTCTA
TCTCTCTCATCAATCGATTGGTAAAGGAACCTTTGGATCGAGTTCCGATCTGGTTGATTG
TTCTTCGATCTGTGTCATCGGTCTTGTGCGGCGCATATGGATATAGGGTTCTTCGTTTGT
GTCATAGGAACAAATGAGAAATGATAGGATTTAAGTTAGTTTGAGATGAGTATTAAGTGG
AGTGTAGTATATAGAGACTCTCAGCTAGAANAAGTCTTCCAGGTATCAAATTAGTATTGT
TGACAAATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGT
   
Sequence Name 41-K116054-SALK_022835C-R210
GenBank Accession LN485315 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116054-SALK_022835C-R210
CACCACTTATATTTGTCAACAATACTAATTTGATACCTGGAAGACTTTTTCTAGCTGAGA
GTCTCTATATACTACTCTCCACTTAATACTCATCTCAAACTAACTTAAATCCTATCATTT
CTCATTTGTTCCTATGACACAAACGAAGAACCCTATATCCATATGCGCCGCATAAGACCG
ATGACACAGATCGAAGAACAATCAACCAGATCGGAACTCGATCCAAAGGTTCCTTTACCA
ATCGATTGATGAGAGAGATAGAAAGAGAGATAGAGTACCAGTAGGTTCCAACGACAGGGG
TAACGAGAAGAGTGGCGCTGATCCAATTCTCAGAGACCTTGTGGTGGATCTTCTCCGGCA
GATCCTTCCAGAGACCGGTCATGATCTTCTGCTGAAACGGAGAAAGTGCGTAAACCACCG
CCTTCAATTTCACCGGCTGCTTCCCCATTTTCTTTTTCTCTTCCCAGATAATCCCAACAA
CAACAATCCACAGTACGAGAACGAATCTCCCCCAAAACCTAAAATAAACAATTCTCAACG
AAGCTTATGTTTTTTATCCGATTCCGGTTCAGTTACAATTACAATTGAACCGGATGACCG
GCTGCAAAATTTATCCTGATTTGTTTCGGTTCGATCCGGTTTGAGTAAACCGAAATAGAT
TAATCACTAGTCCAGACATAAATGATGATGATGATACAGCTAGTGTTCCGGAGGAGAAGG
ACGATCGATCTAAGGAGACGATGAAGATCTTCCACGTTCCTTGTCTGCAAGACAACTACT
CTTATCTGTGAGCCATTTTGATATCCTACTTCAATTTCGTTGCTTCTTAATCTGACTCGG
TTCATCAACA

Last Updated on 06.12.2018 16:11