Sequences from confirmation amplicon


Confirmation sequences for line SALK_024490c and hit At1g68440 are shown.
Line ID SALK_024490c
Locus At1g68440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25658647
Primers used for PCR 75by - LBb3, show primer details
   
Sequence Name 32-K108287-SALK_024490C-75by
GenBank Accession LN485161 [GenBank]
Sequencing primer gene specific primer, 75by: 5'-AAATTATCATCAATGATCCGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K108287-SALK_024490C-75by
ATTATTTTGGACCCTCAATCCAACCTTATTTTCATAATTGTGAAAAAAAAGAAAAAAAAA
ATTTATATTGTGGTAGGTGGTTCGAACCACTCAAAAATATTTATGATAATAACACCCAAA
AACAAACTAAACTATTATTTATTTATTGTGGTCATGAGATAGTTACAGACACAGAGAATA
CATCTGTCAAAAAGCTGACTCATTGTATCTTATCTTTCCCATTTCCTCTTACCTTTTTTC
CTACGTGTTGCCACAAAACTCTCATCTTCACCCACTCAATCTCCGCCACGTGTACTCTCT
AACCCTTCAGTTCGGTCTTTTTAAGGCTTCCCCAAGCCTTCGCATCCAAATCTCTCTTCT
TTTTTTCTCTCTTTCTCATAACAACAAAAAAACAAAAAAAAACCCTATTTAGTTTTTTCT
TTCGGCAAAAATTATGGAGGTCCCGGGGATTAATAAAATAAAAAATTCCAAATTGGGTAT
AAACTCAATAAAGGATCCTTCGTTTCTTTCTCAATCGGTGGCTGTTCCCGAATTCGAAAA
GCTACCCCAACTTTATGGTTTTGGGAAAGGGGGAGCTTTGATTATCGAATTTTTAGCTAA
TTTCACCAATTTTGTTAGTAAACTCACCAGCCAATTTGTTAGGTAAAAAAAAAAAAATGT
CTCGGTTTCCTCTCCACTTTTTTTGGATGATTACAATACCGATTCCAATGTTTCTTGTTC
CTCAACCG
   
Sequence Name 16-K108286-SALK_024490C-R210
GenBank Accession LN484714 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K108286-SALK_024490C-R210
GCGTCATTTGTTACCCACTCGAACCAATCCCACCAAGATCAGGCCATGAGAACAAATCTC
TTAAACTACCACTATATCGCCTCCCCATCCATGTGCAATTACCATTGTCTCCCTGATCAT
CATCATCATCGTAATAATCAGATCCTCTAACGCGGAAACCTCCGTTGACTCGTCTTCGAT
TAAAGATCGAGTCAACATCCTCATCTTCATCATCAGCCTCATCTTCTTCGTCTTTTTCAT
CGTCAGAGGAGACGGTAGAGGAACAAGAAACATCGGAATCGCTATCGTAATCATCAAAAA
GAGTTGGAGAGGAAACAGAGACATCTATTTTTCTTAACCTAACAACTAGACTGTTGAGTT
TACTAACAAAGTTGGTGAAATAAGCTAGAAATGCGATAATCAAAGCTCCCCATTTCCAAA
AACCATAAGCTTGGTGTAGCTTTCCGATTCCGGAAACAGCAACAGATCGAGAAAGAAACG
AAGGATCATTAATCGAGTTTATACCAACTTCGAAATCTCTTATTCTATTAATCACCGGTA
CCTCCATAATCTCTGCAGAAAGAAAAAACTAATTAGGTTTTTTTTTTGTTTTTTGGTTGT
TAGGAGAAAGAGAGAAAAAAAGAAGAGAGTTTTGGATGCAAAGGCTTGGGGAAGCCTTAA
AAAGACCGAACTGAAGGGTTAGAGAGTACACGTGGCGGAAATTGAGTGGGTGAAAATGAG
AGTTTTGTGGCAACACGTAGGAAAAAAGGTAAAAGGAAATGGGGAAGATAAGATACAATG
AGTCACCTTTCTGACAGATGTATTCCC

Last Updated on 06.12.2018 16:11