Sequences from confirmation amplicon


Confirmation sequences for line SALK_025560 and hit At3g61190 are shown.
Line ID SALK_025560
Locus At3g61190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22651376
Primers used for PCR 28mh - LBb3, show primer details
   
Sequence Name 79-K114279-SALK_025560C-28mh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 28mh: 5'-AAAACCGAATTCAAATGAACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K114279-SALK_025560C-28mh
TTTTTTTTGGTGGCCTTGGCCCTTTTTGGTTGGGCCAACTTCTTTATATCTGGAAAAGGA
CCGGGCGGGAAAAAAAAAAAAAATCCCCAAGCAACTTCTTCCCAAACTCCCTGGCCACCT
CCCAAAGGGAGGTGGCCAAAAAAAAAACTGGTCCTTCCATTCAACTAAAGGATAAAACCA
ACAAAAAACCTTTAACCCCAAAGGATATTTTTGGGTAAACCAACCAACAACCATTAAACA
AATGGACCCTAAAACAACTAAAACAACTAAAAAACCCTTGGCGAACTTTTTAAAGGAACG
GGGGGGGTTTTCCTTTCCACCATTAAAACGGGCAACACCTAATGGCCCTTCACCCCCGGG
CCCTAAAAAATTTCCACCAAGCGGCCCACCCTGGTTGGCCCCACCAGGCAAAAACCCGGT
TGAAGGGGGGTCCC
   
Sequence Name 65-K114278-SALK_025560C-R210
GenBank Accession LN485878 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K114278-SALK_025560C-R210
GCGTCATTTGTATAATGATTGTCGATTGATCTACCAAAATATATCATTTGGGTTTAATGT
TTATTTGTTGGTCTAATCATTAAGTAGAATGAAAGAACAAGTTTATATATAGACAACATC
ACTTAGGAAGGTGACAAGGAAGTTTGGAAAGAAGTCGCTCGGAGATTTCTTCTCTTTTTC
CCGCCAGCTCATTATCAAGATATAAAGAAGTCGACCAAACAAAAAAGGGCAAAGACAACA
AAAAAAAAAAACAGGGAATTTACTTTTAATTTTAAAATAAGTATTTGGTTCATTTGAATT
CGGTT

Last Updated on 06.12.2018 16:11