Sequences from confirmation amplicon


Confirmation sequences for line SALK_026163 and hit At1g19250 are shown.
Line ID SALK_026163
Locus At1g19250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6651326
Primers used for PCR 39ke - LBb3, show primer details
   
Sequence Name 52-K113814-SALK_026163C-R210
GenBank Accession LN485562 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113814-SALK_026163C-R210
TTGCTACGGTTAGATGGCAGAAGAAGCTCAAGCTATTGTTCCTGAACCTTTCCGAACTTG
GCTTGAGTTTCCAAGCGGTGTCATGCCTTTATACAGGTACATACACATAAAAATCGTCTT
TAGATTAATTGAAATTATGTGAAAATGCATTAGTTGCACACTTAAAAAGACGATCATGCT
ATAATATATAGTTCTATGACTATAAACATATTCAGTGGTACAAGTGGAAGCCTATATATT
CTTTTATTAGTATACTCATGAAATTTGGACCGTTGCTTATACAAA
   
Sequence Name 65-K113815-SALK_026163C-39ke
GenBank Accession LN485874 [GenBank]
Sequencing primer gene specific primer, 39ke: 5'-GTTTGTATAAGCAACGGTCCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113815-SALK_026163C-39ke
CTTCACTTGTACACTGAATATGTTTATAGTCATAGAACTATATATTATAGCATGATCGTC
TTTTTAAGTGTGCAACTAATGCATTTTCACATAATTTCAATTAATCTAAAGACGATTTTT
ATGTGTATGTACCTGTATAAAGGCATGACACCGCTTGGAAACTCAAGCCAAGTTCGGAAA
GGTTCAGGAACAATAGCTTTGAGCTTCTTCTTGCCATCATAACCAGTAGCAAGTATCACA
ACATCAGTGATACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
GAA

Last Updated on 06.12.2018 16:11