Sequences from confirmation amplicon


Confirmation sequences for line SALK_026580c and hit At2g22230 are shown.
Line ID SALK_026580c
Locus At2g22230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9450664
Primers used for PCR 44sf - LBb3, show primer details
   
Sequence Name 42-K116586-SALK_026580C-44sf
GenBank Accession LN485337 [GenBank]
Sequencing primer gene specific primer, 44sf: 5'-ACCTCTACCAAATTAGCGAGATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116586-SALK_026580C-44sf
TTTCAGTATCAAAGCTGCAGAAAGCTTTCACACATTTTTGGATCAAGTTTTTAGCAAAGT
GAAGTTTGAAGTCCCCTCAACTATAATATACTCATCACTCTATCAGACCACAGAAGGACA
ATTCAATTACCAACCAGGTGAAGATCTCGCGCTAATCTATCAGGATTCAGAATCCAAACT
AGCAGATCATTAACCTCTTATCATAGTTAACATCCATCTTCGATTAAAACAGTAGCGAAC
ATTAAAGCATTTTGAAAGGAATACAACAGCTAACATATCAATTACAAAATATTTGATCTT
CTTGAGGACAAAAGTTTGCAACTTTAAACGATTATTATTACCTCAATCATGAGAACAATA
TATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTC
   
Sequence Name 25-K116585-SALK_026580C-R210
GenBank Accession LN485010 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116585-SALK_026580C-R210
AAACTTTTGTTCCCAAGAAGATCAAATATTTTGTAATTGATATGTTAGCTGTTGTATTCC
TTTTCAAAATGCTTTAATGTTCGCTACTGTTTTAATCGAAGATGGATGTTAACTATGATA
AGAGGTTAATGATCTGCTAGTTTGGATTCTGAATCCTGATAGATTAGCGCGAGATCTTCA
CCTGGTTGGTAATTGAATTGTCCTTCTGTGGTCTGATAGAGTGATGAGTATATTATAGTT
GAGGGACTTCAACTTCACTTTGCTAAAAACTTGATCCAAAATGTGTGAAGCTATCTGCAG
CTTTGATACTGAAAACTCGAGTCTGCGTAGTAGAAATATGCAACTTTAAGCTTGAAAGAG
GTTAATGATCTCGC

Last Updated on 06.12.2018 16:11