Sequences from confirmation amplicon


Confirmation sequences for line SALK_030355c and hit At1g15720 are shown.
Line ID SALK_030355c
Locus At1g15720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5405917
Primers used for PCR 53ew - LBb3, show primer details
   
Sequence Name 59-K111283-SALK_030355C-53ew
GenBank Accession LN485713 [GenBank]
Sequencing primer gene specific primer, 53ew: 5'-TAAGAAACCTCTTTCGAGCTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K111283-SALK_030355C-53ew
TCTTTGAGAGCTGATAAGATGAGAGATGGCATTGTACCTGATGTTAGTCTCACGAATCTT
CTGGTAAATCTCAGGTTCCTCAAACGCCTTGTTTAGATCACTCTCCCATTTCAACAACTC
ACGAGTCACCAAGTCGCTCTCTTTCTCCTTCAATGTCCCAATCCGATTCCTCCAAATCCT
CTCAAGGGCATCACCGAAGAAGCCATCCGAAGCATTCACTGGCCACATAAACCTAAGCGT
GCATTCCACAGCAGTCCAGCAATACGCAGATTTGAGAGATCCCATGATAACCGAATGTTC
CTGTAACAGAAGCTTCTCAAGAATTTCGAGGAGATCAAGCATTCCTTCGTCTACGGTACC
TTGAATCATGGAATTGGAGATATCCCGCAAAACAGCAGTTAGCTTGAGTTTTACACAGTC
GTCGGAATCTACTGGTTTCAGAGCCGATAACAGTGGAAAAGCGTAAACCCTAGGATTTAG
TTGGCGTCGGAGGAAAAATTCAGCTACCCATTTGTCCATCACTTCCATTTCCAAGACTTT
GCAAGTAAAAGATGCGAAAACCCGCGATCAGTTTTCGCCGTCGGTGCGTGGTTAGGGTTA
CAATTTTGGGAAGAGGAAAAGCTCCGACCAACTATGGTGGCAACAACACACGTGTGAATC
ATTCAAATTTAAAATGTGTTTTTTTCTTTTAATTAAATAAGACGAGACATTCAAGCGGCC
CATTATATAATCGGCCCATAACGAAACGAATTGTGGTGTAAACAAATTGACGCTNAGACA
ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
AGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
   
Sequence Name 43-K111282-SALK_030355C-R210
GenBank Accession LN485353 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K111282-SALK_030355C-R210
CATTTGTTACCCACAATTCGTTTCGTTATGGGCCGATTATATAATGGGCCGCTTGAATGT
CTCGTCTTATTTAATTAAAAGAAAAAAACACATTTTAAATTTGAATGATTCACACGTGTG
TTGTTGCCACCATAGTTGGTCGGAGCTTTTCCTCTTCCCAAAATTGTAACCCTAACCACG
CACCGACGGCGAAAACTGATCGCGGGTTTTCGCATCTTTTACTTGCAAAGTCTTGGAAAT
GGAAGTGATGGACAAATGGGTAGCTGAATTTTTCCTCCGACGCCAACTAAATCCTAGGGT
TTACGCTTTTCCACTGTTATCGGCTCTGAAACCAGTAGATTCCGACGACTGTGTAAAACT
CAAGCTAACTGCTGTTTTGCGGGATATCTCCAATTCCATGATTCAAGGTACCGTAGACGA
AGGAATGCTTGATCTCCTCGAAATTCTTGAGAAGCTTCTGTTACAGGAACATTCGGTTAT
CATGGGATCTCTCAAATCTGCGTATTGCTGGACTGCTGTGGAATGCACGCTTAGGTTTAT
GTGGCCAGTGAATGCTTCGGATGGCTTCTTCGGTGATGCCCTTGAGAGGATTTGGAGGAA
TCGGATTGGGACATTGAAGGAGAAAGAGAGCGACTTGGTGACTCGTGAGTTGTTGAAATG
GGAGAGTGATCTAAACAAGGCGTTTGAGGAACCTGAGATTTACCAGAAGATTCGTGAGAC
TAACATCAGGTACAATGCCATCTCTCATCTTAATCAGCTTCTCAAAGAGCAGTGGGCACT
ACTTGGATGTTCTTCTCTTGAATCAGAAGCTCGA

Last Updated on 06.12.2018 16:11