Sequences from confirmation amplicon


Confirmation sequences for line SALK_032288c and hit At3g24360 are shown.
Line ID SALK_032288c
Locus At3g24360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8841858
Primers used for PCR 64kh - LBb3, show primer details
   
Sequence Name 69-K113914-SALK_032288C-64kh
GenBank Accession LN485981 [GenBank]
Sequencing primer gene specific primer, 64kh: 5'-TTGGCTGTATCAAGGCTTATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113914-SALK_032288C-64kh
GGTTTTTTTGGGTTTCCCTTTCCTTGCATCAAAGGAGTTTTCTTTATCAAATTCACACTG
GATAGAAATTTTTATAACCATGGTCTTAGTTTATCTGCAGGCGGTGATGTGAAGCAGATT
ACATCCAAAAATCAATTGTCAGATATGATAGAGGTCTGGTTGTGCAATTGTTTCCCCCAT
AAGTCAGGATATGATGATTACTGAAACAGAAATAGAGAAATACTACTTCTACTGAATGTA
AACAAATAACACATTGGGGCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 58-K113913-SALK_032288C-R210
GenBank Accession LN485692 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113913-SALK_032288C-R210
GTAATCATCATATCCCGACTTACGGGGGAAACAATCGCACAACCAGACCTCTATCATATC
TGACAATCGATTTTTGGATGTAATCTGCTTCACATCACCGCCTGCAGATAAACTAAGACC
ATGGTTATAAAAATTTCTATCCAGTGTGAATTTGATAAAGAAAACTCCTTTGATGCAAGA
AAGGGAAACCCAAAAAACCTCACATAGAACAAAACACAGAGTACAAAAATACCAAAGAGT
TGCAAAATGACATTTTGTATGTTTAAAGAAAGAGATAAGCCTCGTTTCCAGCC

Last Updated on 06.12.2018 16:11