Sequences from confirmation amplicon


Confirmation sequences for line SALK_035555c and hit At5g04710 are shown.
Line ID SALK_035555c
Locus At5g04710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1359363
Primers used for PCR 02ca - LBb3, show primer details
   
Sequence Name 89-K108074-SALK_035555C-02ca
GenBank Accession LN486325 [GenBank]
Sequencing primer gene specific primer, 02ca: 5'-AAAACGAAGACTGGAATCTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K108074-SALK_035555C-02ca
GGAACTAGTTCAAGCTTTGATAATTGAAGGGTTCTTCACAAAAATCGTTTTTTTTTTCTT
TCTTAAATCCCTTATTTGTTGGGTTCAGGGAACCTACATTTGTTCCTCATTTTGAAGGTA
TGTGCCTGGTAATGGTTTTCATGCCATAGCTGCTCACACCGATAGCCCGTGTCTTAAACT
TAAACCGAAGTCTGCTTCATCCAAGTCTGGCTATCTCATGGTGAATGTTCAGGATATATT
GTGGTGTAAACAAATTGCCGCTTAAACAACTTAATAACCCATTGCGGACGTTTTTAATGT
ACTGGGGGGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCTTTCACCCCC
TGGCCCTGAAAAAGTTGCACCAAGCGGTCCACCCTGGTTTGCCCCATCAGGCAAAAATCC
TGTTTGATGGTGGTTCCAAA
   
Sequence Name 80-K108073-SALK_035555C-R210
GenBank Accession LN486217 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K108073-SALK_035555C-R210
GTCATTTGTTACACACAATATATCCTGAACATTCACCATGAGATAGCCAGACTTGGATGA
AGCAGACTTCGGTTTAAGTTTAAGACACGGGCTATCGGTGTGAGCAGCTATGGCATGAAA
ACCATTACCAGGCACATACCTACAAAATGAGGAACAAATGTAGGTTCCCTGAACCCAACA
AATAAGGAATCTAAGAAAGAAAAAAAAAACGATTTTTGTGAAAAACCCTTCAATTATCAA
AGCTTGAACTAGTTCCAGGATTCATTATCAAAGCCAAACCAAATCTTATACCATGGAAGC
ATAATTGAATCGGAAGACTCACTTTTCTCCGACAGCAAAAGCAACCAAACAGGACATGTT
TCGTGTAAAGAAGTAACGGCCACCAGGTTTCAAATTCCAGTCTTCGTT
Primers used for PCR CE68 - LBb3, show primer details
   
Sequence Name 78-K119133-SALK_035555C-CE68
GenBank Accession LN486202 [GenBank]
Sequencing primer gene specific primer, CE68: 5'-TTCATCTGATTTTGTTGCCAGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K119133-SALK_035555C-CE68
GTTAATCCTCACTGGTTCCTGGTGCTAAACAATACACGAAGAACAAAACAGCATGAATGA
GCTTCAAAACATAACTCCACTTGATGGCAAATGCATTAGCCTAAAACTTCACTAGACAAG
CTCATGAAAGAAATTTACCGATCAAGATGAATGGCCAAAGTGGGTACCCTTAGTAATGGC
CTTTTCACTTTGACAAGCCTATGAACAAACGAGCCATCACTGGCTCTCACAATTGCTCGC
CCCGCAACACTTAAGTCTCGATCAAACCATGTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
T
   
Sequence Name 64-K119132-SALK_035555C-R210
GenBank Accession LN485860 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K119132-SALK_035555C-R210
ACCACATGGNTTTGATCGAGACTTAAGTGTTGCGGGGCGAGCAATTGTGAGAGCCAGTGA
TGGCTCGNTTTGTTCATAGGCTTGTCAAAGTGAAAAGGCCATTACTAAGGGTACCCACTT
TGGCCATTCATCTTGATCGGTAAATTTCTTTCATGAGCTTGTCTAGTGAAGTTTTAGGCT
AATGCATTTGCCATCAAGTGGAGTTATGTTTTGAAGCTCATTCATGCTGTTTTGTTCTTC
GTGTATTGTTTAGCACAGTGAACAGTGATGGATTTAAACCAAATTTGGAGACCCAACTTG
TTCCGCTACTGGCAACAAAATCAGATGA

Last Updated on 06.12.2018 16:11