Sequences from confirmation amplicon


Confirmation sequences for line SALK_038916c and hit At5g67600 are shown.
Line ID SALK_038916c
Locus At5g67600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26959868
Primers used for PCR 54ew - LBb3, show primer details
   
Sequence Name 60-K111283-SALK_038916C-54ew
GenBank Accession LN485740 [GenBank]
Sequencing primer gene specific primer, 54ew: 5'-GCGATAGTCTCTTTCATTCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K111283-SALK_038916C-54ew
GAGTTTTACCTAACCCCATGATTGTTCATCATCTAACATCTAACTGCAGGTCAAGTCTTA
CTCGGACAACTTCAAGTGGAAAGGCAATCCTCAACCAGAAAACTGATGTTACACCTTCTT
CCAATTCTTCTCGAGCAAAAGAAGAAACCTCATTTGATATTAATTCTCATGTTTCTTTGT
TATTTTAGCATCTGTGAGCTTTTTGGTTGAAAAGAACCATTATCCATGGCTATGTTTACT
TATTACAATAATATGATGATGATGATGAAACACACTCCTTTGATATTTCATCAAGATTTC
TGATCACTTCTTTACTGGTTTATTTCGAATTCGTGTGATTGGATTATGAAAGAGAGAGAT
AATTATGGTCGGAGTCAATAAAACATAAGATACCAAAATTCGAATTGAAAGAAGATAACA
ACAGAAGAAAGATTGTTGAACATTACATAACATATATGAGGAAATAAAAGTTGACTGAGG
CAACGTATATAATTATCCTCCGATCAGAAACATGCATCCAAGAGACAGCAACAACAGAGC
GCAGCCAAACTGTGACGTATTTATGAACAGAGCAAAGACATATATTAACTAATACAGTAT
TATTCCACTAAAACCTAATAACACATTGCGGACAAATAACAAAAAATACCGCAATAACAC
ATTAATAACACATTGCGGAAAATATTTGTTTGATACAAATTGCGTCAAGTTGTCCGCAGG
ATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTT
   
Sequence Name 44-K111282-SALK_038916C-R210
GenBank Accession LN485373 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K111282-SALK_038916C-R210
CATTTGTTTACCCCAATATATCCTGCGGACAACTTGACGCAATTTGTATCAAACAAATAT
TTTCCGCAATGTGTTATTAATGTGTTATTGCGGTATTTTTTGTTATTTGTCCGCAATGTG
TTATTAGGTTTTAGTGGAATAATACTGTATTAGTTAATATATGTCTTTGCTCTGTTCATA
AATACGTCACAGTTTGGCTGCGCTCTGTTGTTGCTGTCTCTTGGATGCATGTTTCTGATC
GGAGGATAATTATATACGTTGCCTCAGTCAACTTTTATTTCCTCATATATGTTATGTAAT
GTTCAACAATCTTTCTTCTGTTGTTATCTTCTTTCAATTCGAATTTTGGTATCTTATGTT
TTATTGACTCCGACCATAATTATCTCTCTCTTTCATAATCCAATCACACGAATTCGAAAT
AAACCAGTAAAGAAGTGATCAGAAATCTTGATGAAATATCAAAGGAGTGTGTTTCATCAT
CATCATCATATTATTGTAATAAGTAAACATAGCCATGGATAATGGTTCTTTTCAACCAAA
AAGCTCACAGATGCTAAAATAACAAAGAAACATGAGAATTAATATCAAATGAGGTTTCTT
CTTTTGCTCGAGAAGAATTGGAAGAAGGTGTAACATCAGTTTTCTGGTTGAGGATTGCCT
TTCCACTTGAAGTTGTCCGAGTAAGACTTGACCTGCAGTTAGATGTTAGATGATGAACAA
TCATGGGGTTAGGTTAAAACTTCAATCTAAAGAATGTTCTACCAAATAAATCANAGGAAT
GAAA

Last Updated on 06.12.2018 16:11