Sequences from confirmation amplicon


Confirmation sequences for line SALK_041461c and hit At2g28725 are shown.
Line ID SALK_041461c
Locus At2g28725 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12328888
Primers used for PCR 11tw - LBb3, show primer details
   
Sequence Name 14-K116922-SALK_041461C-11tw
GenBank Accession LN484677 [GenBank]
Sequencing primer gene specific primer, 11tw: 5'-TGTAAGAGTCCTCTCTCCCACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116922-SALK_041461C-11tw
CTCAAATCTTTTTACTTCACACACACAAAACTAAACACTTCTATAATTTTGATTCTTTCA
ATGGAACCAATTCTTGAAAAAACCTCAACACGTTGGGAAGTTCTTGTTCAAACAGCAACA
TGGACAATTCTCCTAATGATCACAGTGGCTTTGGCTTCATTTGCTCCGGAGATGGCGTTT
GTGTCGAAATTAAAGTCATCTTCCGATGGGTTCGTTAGGATTCCGATGGATTTACCGGGA
GAAATGTTAATCTTGCCGTCAGAAATGGTGAAGAATTCATATTTAGACGTTTTTTTACCA
ACGATATTCGCAGGAGTTATGGTCATTGCTTCTGTTTCTTTATTGTGGTGTAAACAAATT
GACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
CG
   
Sequence Name 03-K116921-SALK_041461C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116921-SALK_041461C-R210
CTCCAATAAAGTCACTTCCTCGATCAATCCTATCGCCTC

Last Updated on 06.12.2018 16:11